Literature summary for 6.1.1.26 extracted from

Nguyen, D.P.; Lusic, H.; Neumann, H.; Kapadnis, P.B.; Deiters, A.; Chin, J.W.
Genetic encoding and labeling of aliphatic azides and alkynes in recombinant proteins via a pyrrolysyl-tRNA Synthetase/tRNA(CUA) pair and click chemistry (2009), J. Am. Chem. Soc., 131, 8720-8721.

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Application

Application	Comment	Organism
synthesis	synthesis and genetic incorporation of aliphatic azides and alkynes into proteins using the natural pyrrolysyl tRNA synthetase/tRNACUA pair and the efficient bio-orthogonal labeling of these amino acids using [3+2] cycloaddition, i.e. click chemistry. Escherichia coli transformed with pBKPylS10 encoding pyrrolysyl tRNA synthetase and pMyo4TAGPylT-his610 encoding tRNACUA and a C-terminally hexahistidine tagged myoglobin gene with an amber codon at position 4 incorporates (2S)-2-amino-6-[[(prop-2-yn-1-yloxy)carbonyl]amino]hexanoic acid. The yield of protein containing (2S)-2-amino-6-[[(prop-2-yn-1-yloxy)carbonyl]amino]hexanoic acid is not improved by efforts to evolve the enzyme but is increased 5fold by increasing the concentration of (2S)-2-amino-6-[[(prop-2-yn-1-yloxy)carbonyl]amino]hexanoic acid 7.5fold	Methanosarcina barkeri

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Methanosarcina barkeri

Organism

Organism	UniProt	Comment	Textmining
Methanosarcina barkeri	-	-	-

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Release 2023.1 (January 2023)