Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.26 extracted from

  • Mukai, T.; Kobayashi, T.; Hino, N.; Yanagisawa, T.; Sakamoto, K.; Yokoyama, S.
    Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases (2008), Biochem. Biophys. Res. Commun., 371, 818-822.
    View publication on PubMed
Search for more literature for 6.1.1.26

Cloned(Commentary)

Cloned (Comment) Organism
expression of tRNAPyl in Chinese hamster ovary cells. ZLysRS-tRNAPyl pair and GRB2(Am111)-FLAG expressed in HEK293 c-18 cells Methanosarcina mazei

Protein Variants

Protein Variants Comment Organism
L309A/C348V designated as ZLysRS has the L309A and C348V substitutions at the pyrrolysine binding pocket and three mutations at the other sites Methanosarcina mazei
additional information four substitutions (L305I, Y306F, L309A, and C348F) at the binding pocket out of the six mutations found in AcLysRS from Methanosarcina barkeri are transplanted into Methanosarcina mazei PylRS to create mAcLysRS Methanosarcina mazei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-pyrrolysine + tRNAPyl Methanosarcina mazei
-
 AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina mazei
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Boc-lysine + tRNAPyl Nepsilon-tert-butyloxycarbonyl-L-lysine Methanosarcina mazei ?
-
 ?
ATP + L-pyrrolysine + tRNAPyl
-
 Methanosarcina mazei AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
ATP + N-alpha-acetyl-L-lysine + tRNAPyl
-
 Methanosarcina mazei ?
-
 ?
ATP + N-alpha-benzyloxycarbonyl-L-lysine + tRNAPyl
-
 Methanosarcina mazei ?
-
 ?

Synonyms

Synonyms Comment Organism
pyrrolysyl-tRNA synthetase
-
 Methanosarcina mazei