Literature summary for 6.1.1.24 extracted from

Nureki, O.; ODonoghue, P.; Watanabe, N.; Ohmori, A.; Oshikane, H.; Araiso, Y.; Sheppard, K.; Soell, D.; Ishitani, R.
Structure of an archaeal non-discriminating glutamyl-tRNA synthetase: a missing link in the evolution of Gln-tRNAGln formation (2010), Nucleic Acids Res., 38, 7286-7297.

Cloned(Commentary)

Cloned (Comment)	Organism
ND-GluRS, phylogenetic analysis	Methanothermobacter thermautotrophicus

Crystallization (Commentary)

Crystallization (Comment)	Organism
full-length ND-GluRS encompassing residues 1-552, including four cysteines and 18 methionine residues, hanging-drop method by mixing 0.001 ml of reservoir buffer containing 50 mM sodium cacodylate, pH 7.0, 50 mM calcium chloride and 8% PEG 6000, with 0.001 ml of protein solution at 20 mg/ml, 20°C, 5 days, X-ray diffraction structure determination and analysis, single-wavelength anomalous dispersion method, using sulfur anomalous dispersion, modelling	Methanothermobacter thermautotrophicus

Crystallization (Comment)

Organism

full-length ND-GluRS encompassing residues 1-552, including four cysteines and 18 methionine residues, hanging-drop method by mixing 0.001 ml of reservoir buffer containing 50 mM sodium cacodylate, pH 7.0, 50 mM calcium chloride and 8% PEG 6000, with 0.001 ml of protein solution at 20 mg/ml, 20°C, 5 days, X-ray diffraction structure determination and analysis, single-wavelength anomalous dispersion method, using sulfur anomalous dispersion, modelling

Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
63237	-	x * 63237, sequence calculation	Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-glutamate + tRNAGlx	Methanothermobacter thermautotrophicus	-	AMP + diphosphate + glutamyl-tRNAGlx	-	?
additional information	Methanothermobacter thermautotrophicus	ND-GluRS recognizes both tRNAGlu and tRNAGln without significantly discriminating between them, tRNA discrimination module, overview. The first point of significant distinction between the GlnRS and ND-GluRS tRNA recognition involves residues contacting the G36 nucleobase. A second distiction involves a beta-hairpin module in the CP domain	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Methanothermobacter thermautotrophicus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-glutamate + tRNAGlx	-	Methanothermobacter thermautotrophicus	AMP + diphosphate + glutamyl-tRNAGlx	-	?
additional information	ND-GluRS recognizes both tRNAGlu and tRNAGln without significantly discriminating between them, tRNA discrimination module, overview. The first point of significant distinction between the GlnRS and ND-GluRS tRNA recognition involves residues contacting the G36 nucleobase. A second distiction involves a beta-hairpin module in the CP domain	Methanothermobacter thermautotrophicus	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 63237, sequence calculation	Methanothermobacter thermautotrophicus
More	structure analysis, and comparison with the structure of the Escherichia coli GlnRS-tRNAGln complex and with GluRS structure, structural determinants responsible for specific tRNAGln recognition, overview	Methanothermobacter thermautotrophicus

Synonyms

Synonyms	Comment	Organism
ND-GluRS	-	Methanothermobacter thermautotrophicus
non-discriminating glutamyl-tRNA synthetase	-	Methanothermobacter thermautotrophicus

General Information

General Information	Comment	Organism
evolution	evolution in the GlxRS family, overview. Two pathways exist for the formation of Gln-tRNAGln. The evolutionarily older indirect route utilizes a non-discriminating glutamyl-tRNA synthetase, ND-GluRS, that can form both Glu-tRNAGlu and Glu-tRNAGln. The Glu-tRNAGln is then converted to Gln-tRNAGln by an amidotransferase. ND-GluRS is the evolutionary predecessor of both the glutaminyl-tRNA synthetase, GlnRS, and the eukaryotic discriminating GluRS	Methanothermobacter thermautotrophicus