Literature summary for 6.1.1.24 extracted from

Schulze, J.O.; Masoumi, A.; Nickel, D.; Jahn, M.; Jahn, D.; Schubert, W.D.; Heinz, D.W.
Crystal structure of a non-discriminating glutamyl-tRNA synthetase (2006), J. Mol. Biol., 361, 888-897.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of C-terminally and N-terminally His6-tagged ND-GluRS in Escherichia coli strain BL21	Thermosynechococcus vestitus

Crystallization (Commentary)

Crystallization (Comment)	Organism
GluRS in complex with glutamate, hanging drop vapor diffusion at 20°C, 0.003 ml protein solution containing 3 mg/ml protein in 20 mM HEPES, pH 7.9, 20 mM NaCl, 10 mM DTT, 0.25 mM zinc acetate and 0.25 mM MgCl2, is mixed with 0.003 ml reservoir solution containing 740 mM sodium citrate, 140 mM citric acid, pH 5.8, and 10 mM DTT, 1-2 weeks, cryoprotection using 25% v/v of a 50% w/v trehalose solution, X-ray diffraction structure determination and analysis at 2.45 A resolution	Thermosynechococcus vestitus

Crystallization (Comment)

Organism

GluRS in complex with glutamate, hanging drop vapor diffusion at 20°C, 0.003 ml protein solution containing 3 mg/ml protein in 20 mM HEPES, pH 7.9, 20 mM NaCl, 10 mM DTT, 0.25 mM zinc acetate and 0.25 mM MgCl2, is mixed with 0.003 ml reservoir solution containing 740 mM sodium citrate, 140 mM citric acid, pH 5.8, and 10 mM DTT, 1-2 weeks, cryoprotection using 25% v/v of a 50% w/v trehalose solution, X-ray diffraction structure determination and analysis at 2.45 A resolution

Thermosynechococcus vestitus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
additional information	-	additional information	kinetics	Thermosynechococcus vestitus
0.00079	-	tRNAGlu	-	Thermosynechococcus vestitus
0.0037	-	tRNAGln	-	Thermosynechococcus vestitus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Thermosynechococcus vestitus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-glutamate + tRNAGln	Thermosynechococcus vestitus	tRNAGln is initially mischarged with glutamate by a non-discriminating glutamyl-tRNA synthetase	AMP + diphosphate + L-glutamyl-tRNAGln	-	?
ATP + L-glutamate + tRNAGlu	Thermosynechococcus vestitus	-	AMP + diphosphate + L-glutamyl-tRNAGlu	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermosynechococcus vestitus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant C-terminally and N-terminally His6-tagged ND-GluRS from Escherichia coli strain BL21 by nickel affinity and anion exchange chromatography, and gel filtration to homogeneity	Thermosynechococcus vestitus

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + L-glutamate + tRNAGlx = AMP + diphosphate + L-glutamyl-tRNAGlx	active site structure and substrate recognition	Thermosynechococcus vestitus	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + L-glutamate + tRNAGln	tRNAGln is initially mischarged with glutamate by a non-discriminating glutamyl-tRNA synthetase	Thermosynechococcus vestitus	AMP + diphosphate + L-glutamyl-tRNAGln	-	?
ATP + L-glutamate + tRNAGlu	-	Thermosynechococcus vestitus	AMP + diphosphate + L-glutamyl-tRNAGlu	-	?
ATP + L-glutamate + tRNAGlu	preferred tRNA substrate	Thermosynechococcus vestitus	AMP + diphosphate + L-glutamyl-tRNAGlu	-	?
additional information	the non-discriminating enzyme charges both tRNAGlu and tRNAGln with glutamate, anticodons of tRNAGlu, 34C/UUC36, and tRNAGln, 34C/UUG36, differ only in base 36, residue Gly366 is responsible for allowing both cytosine and the bulkier purine base G36 of tRNAGln to be tolerated, glutamate recognition structure, overview	Thermosynechococcus vestitus	?	-	?

Subunits

Subunits	Comment	Organism
More	structure comparison of non-discriminating GluRS versus the discriminating GluRS, EC 6.1.1.17, overview	Thermosynechococcus vestitus

Synonyms

Synonyms	Comment	Organism
ND-GluRS	-	Thermosynechococcus vestitus
non-discriminating glutamyl-tRNA synthetase	-	Thermosynechococcus vestitus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.036	-	tRNAGln	-	Thermosynechococcus vestitus
0.1	-	tRNAGlu	-	Thermosynechococcus vestitus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Thermosynechococcus vestitus