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Literature summary for 6.1.1.24 extracted from

  • Lapointe, J.; Duplain, L.; Proulx, M.
    A single glutamyl-tRNA synthetase aminoacylates tRNAGlu and tRNAGln in Bacillus subtilis and efficiently misacylates Escherichia coli tRNA1Gln in vitro (1986), J. Bacteriol., 165, 88-93.
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00045
-
 tRNAGln homologous tRNAGln, in absence of regulatory factor beta Bacillus subtilis
0.00065
-
 tRNAGln homologous tRNAGln, in presence of regulatory factor beta Bacillus subtilis
0.00083
-
 tRNAGlu homologous tRNAGlu, in absence of regulatory factor beta Bacillus subtilis
0.0014
-
 tRNAGlu homologous tRNAGlu, in presence of regulatory factor beta Bacillus subtilis
0.0015
-
 tRNAGln tRNAGln Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + tRNAGlx Bacillus subtilis the enzyme is responsible for the in vivo aminoacylation of both tRNAGlu and tRNAGln in Bacillus subtilis AMP + diphosphate + Glu-tRNAGlx
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + tRNAGln the enzyme aminoacylates tRNAGLU and tRNASGln in Bacillus subtilis and efficiently misacylates Escherichia coli tRNA1Gln in vitro Bacillus subtilis AMP + diphosphate + Glu-tRNAGln
-
 ?
ATP + L-glutamate + tRNAGlu the enzyme aminoacylates tRNAGlu and tRNAsGln in Bacillus subtilis and efficiently misacylates Escherichia coli tRNA1Gln in vitro, not tRNA2Gln from Escherichia coli or tRNAGlu from Escherichia coli Bacillus subtilis AMP + diphosphate + Glu-tRNAGlu
-
 ?
ATP + L-glutamate + tRNAGlu the enzyme interacts with the G64-C50 or G64-U50 in the Tpsi stem of its tRNA substrate Bacillus subtilis AMP + diphosphate + Glu-tRNAGlu
-
 ?
ATP + L-glutamate + tRNAGlx the enzyme is responsible for the in vivo aminoacylation of both tRNAGlu and tRNAGln in Bacillus subtilis Bacillus subtilis AMP + diphosphate + Glu-tRNAGlx
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
 tRNAGln homologous tRNAGln Bacillus subtilis
0.05
-
 tRNAGlu homologous tRNAGlu Bacillus subtilis
0.07
-
 tRNAGln tRNAGln from E. coli Bacillus subtilis