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Literature summary for 6.1.1.23 extracted from

  • Alperstein, A.; Ulrich, B.; Garofalo, D.M.; Dreisbach, R.; Raff, H.; Sheppard, K.
    The predatory bacterium Bdellovibrio bacteriovorus aspartyl-tRNA synthetase recognizes tRNAAsn as a substrate (2014), PLoS ONE, 9, e110842 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bdellovibrio bacteriovorus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 tRNAAsp at pH 7.2 and 37°C Bdellovibrio bacteriovorus
0.0014
-
 tRNAAsn at pH 7.2 and 37°C Bdellovibrio bacteriovorus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate + tRNAAsp Bdellovibrio bacteriovorus
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
ATP + L-aspartate + tRNAAsp Bdellovibrio bacteriovorus HD100
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?

Organism

Organism UniProt Comment Textmining
Bdellovibrio bacteriovorus Q6MI59
-
-
Bdellovibrio bacteriovorus HD100 Q6MI59
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-affinity column chromatography Bdellovibrio bacteriovorus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + tRNAAsn
-
 Bdellovibrio bacteriovorus AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
ATP + L-aspartate + tRNAAsn
-
 Bdellovibrio bacteriovorus HD100 AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
ATP + L-aspartate + tRNAAsp
-
 Bdellovibrio bacteriovorus AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
ATP + L-aspartate + tRNAAsp
-
 Bdellovibrio bacteriovorus HD100 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?

Synonyms

Synonyms Comment Organism
Aspartyl-tRNA synthetase
-
 Bdellovibrio bacteriovorus
AspRS
-
 Bdellovibrio bacteriovorus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.17
-
 tRNAAsn at pH 7.2 and 37°C Bdellovibrio bacteriovorus
0.52
-
 tRNAAsp at pH 7.2 and 37°C Bdellovibrio bacteriovorus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Bdellovibrio bacteriovorus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
120
-
 tRNAAsn at pH 7.2 and 37°C Bdellovibrio bacteriovorus
400
-
 tRNAAsp at pH 7.2 and 37°C Bdellovibrio bacteriovorus