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Literature summary for 6.1.1.23 extracted from

  • Mladenova, S.R.; Stein, K.R.; Bartlett, L.; Sheppard, K.
    Relaxed tRNA specificity of the Staphylococcus aureus aspartyl-tRNA synthetase enables RNA-dependent asparagine biosynthesis (2014), FEBS Lett., 588, 1808-1812 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
tRNAAsp at pH 7.2 and 37°C Staphylococcus aureus
0.0031
-
tRNAAsn at pH 7.2 and 37°C Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate + tRNAAsp Staphylococcus aureus
-
AMP + diphosphate + L-aspartyl-tRNAAsp
-
?
ATP + L-aspartate + tRNAAsp Staphylococcus aureus FPR3757
-
AMP + diphosphate + L-aspartyl-tRNAAsp
-
?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-
Staphylococcus aureus FPR3757
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + tRNAAsn
-
Staphylococcus aureus AMP + diphosphate + L-aspartyl-tRNAAsn
-
?
ATP + L-aspartate + tRNAAsn
-
Staphylococcus aureus FPR3757 AMP + diphosphate + L-aspartyl-tRNAAsn
-
?
ATP + L-aspartate + tRNAAsp
-
Staphylococcus aureus AMP + diphosphate + L-aspartyl-tRNAAsp
-
?
ATP + L-aspartate + tRNAAsp
-
Staphylococcus aureus FPR3757 AMP + diphosphate + L-aspartyl-tRNAAsp
-
?

Synonyms

Synonyms Comment Organism
Aspartyl-tRNA synthetase
-
Staphylococcus aureus
AspRS
-
Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
tRNAAsn at pH 7.2 and 37°C Staphylococcus aureus
0.24
-
tRNAAsp at pH 7.2 and 37°C Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Staphylococcus aureus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
70
-
tRNAAsn at pH 7.2 and 37°C Staphylococcus aureus
100
-
tRNAAsp at pH 7.2 and 37°C Staphylococcus aureus