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Literature summary for 6.1.1.22 extracted from
- Soluble expression and purification of a full-length asparaginyl tRNA synthetase from Fasciola gigantica (2018), Protein Expr. Purif., 143, 9-13 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli M15 cells | Fasciola gigantica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 132000 | - |
gel filtration | Fasciola gigantica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-asparagine + tRNAAsn | Fasciola gigantica | - |
AMP + diphosphate + L-asparaginyl-tRNAAsn | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fasciola gigantica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Fasciola gigantica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-asparagine + tRNAAsn | - |
Fasciola gigantica | AMP + diphosphate + L-asparaginyl-tRNAAsn | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 61000, His6-tagged enzyme, SDS-PAGE | Fasciola gigantica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| asparaginyl tRNA synthetase | - |
Fasciola gigantica |
| NRS | - |
Fasciola gigantica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Fasciola gigantica | |
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Release 2023.1 (January 2023)
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