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Literature summary for 6.1.1.22 extracted from

  • Filisetti, D.; Theobald-Dietrich, A.; Mahmoudi, N.; Rudinger-Thirion, J.; Candolfi, E.; Frugier, M.
    Aminoacylation of Plasmodium falciparum tRNAAsn and insights in the synthesis of asparagine repeats (2013), J. Biol. Chem., 288, 36361-36371.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.22

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 tRNAAsn in 50 mM HEPES-NaOH, pH 7.2, 10 mM MgCl2, at 37°C Plasmodium falciparum
0.04
-
 L-asparagine in 50 mM HEPES-NaOH, pH 7.2, 10 mM MgCl2, at 37°C Plasmodium falciparum
0.74
-
 ATP in 50 mM HEPES-NaOH, pH 7.2, 10 mM MgCl2, at 37°C Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Plasmodium falciparum 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
127000
-
 2 * 127000, calculated from amino acid sequence Plasmodium falciparum
295000
-
 dynamic light scattering Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-asparagine + tRNAAsn Plasmodium falciparum
-
 AMP + diphosphate + L-asparaginyl-tRNAAsn
-
 ?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-asparagine + tRNAAsn
-
 Plasmodium falciparum AMP + diphosphate + L-asparaginyl-tRNAAsn
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 127000, calculated from amino acid sequence Plasmodium falciparum

Synonyms

Synonyms Comment Organism
AsnRS
-
 Plasmodium falciparum
Asparaginyl-tRNA synthetase
-
 Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 tRNAAsn in 50 mM HEPES-NaOH, pH 7.2, 10 mM MgCl2, at 37°C Plasmodium falciparum
4.2
-
 L-asparagine in 50 mM HEPES-NaOH, pH 7.2, 10 mM MgCl2, at 37°C Plasmodium falciparum
10.1
-
 ATP in 50 mM HEPES-NaOH, pH 7.2, 10 mM MgCl2, at 37°C Plasmodium falciparum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2
-
 tRNAAsn in 50 mM HEPES-NaOH, pH 7.2, 10 mM MgCl2, at 37°C Plasmodium falciparum
13.6
-
 ATP in 50 mM HEPES-NaOH, pH 7.2, 10 mM MgCl2, at 37°C Plasmodium falciparum
105
-
 L-asparagine in 50 mM HEPES-NaOH, pH 7.2, 10 mM MgCl2, at 37°C Plasmodium falciparum