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Literature summary for 6.1.1.22 extracted from

  • Anselme, J.; Härtlein, M.
    Tyr-426 of the Escherichia coli asparaginyl-tRNA synthetase, an amino acid in a C-terminal conserved motif, is involved in ATP binding (1991), FEBS Lett., 280, 163-166.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
Y426F wild-type and mutant enzymes Y426F and Y426S. Y426S replacement leads to a 15fold increase in Km for ATP, while all the other kinetic parameters remain unchanged. Y426F replacement does not affect the kinetic behaviour of the enzyme Escherichia coli
Y426S wild-type and mutant enzymes Y426F and Y426S. Y426S replacement leads to a 15fold increase in Km for ATP, while all the other kinetic parameters remain unchanged. Y426F replacement does not affect the kinetic behaviour of the enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0153
-
Asn wild-type, ATP-diphosphate exchange Escherichia coli
0.0225
-
Asn mutant Y426F, ATP-diphosphate exchange Escherichia coli
0.0306
-
ATP mutant Y426F, aminoacylation Escherichia coli
0.0323
-
Asn wild-type, aminoacylation Escherichia coli
0.0326
-
Asn mutant Y426F, aminoacylation Escherichia coli
0.0336
-
Asn mutant Y426S, aminoacylation Escherichia coli
0.0383
-
Asn mutant Y426S, ATP-diphosphate exchange Escherichia coli
0.076
-
ATP ATP, , wild-type, aminoacylation Escherichia coli
0.245
-
ATP mutant Y426S, aminoacylation Escherichia coli
0.5
-
ATP ATP, , wild-type, mutant Y426F, ATP-diphosphate exchange Escherichia coli
7
-
ATP mutant Y426S, ATP-diphosphate exchange Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
wild-type and mutant enzymes Y426F and Y426S
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-asparagine + tRNAAsn
-
Escherichia coli AMP + diphosphate + L-asparaginyl-tRNAAsn
-
?
additional information ATP-diphosphate exchange Escherichia coli ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
ATP mutant Y426S, aminoacylation Escherichia coli
0.7
-
Asn mutant Y426S, aminoacylation Escherichia coli
1
-
Asn ATP, , mutant Y426F, aminoacylation Escherichia coli
1.3
-
ATP Asn, , mutant Y426S, ATP-diphosphate exchange Escherichia coli
1.3
-
ATP wild-type, aminoacylation Escherichia coli
1.6
-
Asn wild-type, aminoacylation Escherichia coli
2.1
-
ATP mutant Y426S, ATP-diphosphate exchange Escherichia coli
2.2
-
ATP wild-type, 37°C, ATP-diphosphate exchange Escherichia coli
2.3
-
ATP mutant Y426F, ATP-diphosphate exchange Escherichia coli
2.7
-
Asn mutant Y426F, , ATP-diphosphate exchange Escherichia coli
2.8
-
Asn mutant Y426F, aminoacylation Escherichia coli
2.8
-
Asn Asn, , wild-type, ATP-diphosphate exchange Escherichia coli