Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanol | slight stimulation at low tRNA concentrations | Vigna radiata var. radiata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-cyanoalanine | aminoacylation of Asn, competitive | Vigna radiata var. radiata | |
Aspartate 3-hydroxamate | Asn-dependent ATP-diphosphate exchange, competitive | Vigna radiata var. radiata | |
Br- | 1.2 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
CH3COO- | 1.3 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
Cl- | 2.5 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
CNS- | 0.4 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
Cs+ | 1.3 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
I- | 0.5 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
isoasparagine | aminoacylation of Asn, competitive | Vigna radiata var. radiata | |
K+ | 2.5 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
Li+ | 0.7 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
Na+ | 2.0 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
NH4+ | 0.9 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
NO3- | 1.0 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
p-chloromercuribenzoate | - |
Vigna radiata var. radiata | |
threo-3-Hydroxyasparagine | aminoacylation of Asn, competitive | Vigna radiata var. radiata | |
Tris | above 0.3 M | Vigna radiata var. radiata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
Asn | tRNA esterification | Vigna radiata var. radiata | |
0.18 | - |
Asn | ATP-diphosphate exchange | Vigna radiata var. radiata | |
0.26 | - |
isoasparagine | ATP-diphosphate exchange | Vigna radiata var. radiata | |
0.78 | - |
aspartate-3-hydroxamate | ATP-diphosphate exchange | Vigna radiata var. radiata | |
5.8 | - |
3-cyanoalanine | ATP-diphosphate exchange | Vigna radiata var. radiata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates tRNA esterification with 5% of the efficiency compared to Mg2+ | Vigna radiata var. radiata | |
Co2+ | stimulates ATP-diphosphate exchange with 17% the efficiency compared to Mg2+ and tRNA esterification with 45% | Vigna radiata var. radiata | |
Mg2+ | required, optimal concentration is 20 mM in ATP-diphosphate exchange and 12 mM in tRNA esterification, Km: 5.8 mM in ATP-diphosphate exchange, 2.8 in tRNA esterification | Vigna radiata var. radiata | |
Mn2+ | stimulates ATP-diphosphate exchange with 30% the efficiency compared to Mg2+ and tRNA esterification with 72% efficiency | Vigna radiata var. radiata | |
Ni2+ | stimulates tRNA esterification with 15% of the efficiency compared to Mg2+ | Vigna radiata var. radiata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vicia faba | - |
- |
- |
Vicia sativa | - |
- |
- |
Vigna radiata var. radiata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vigna radiata var. radiata |
- |
Vicia faba |
- |
Vicia sativa |
Renatured (Comment) | Organism |
---|---|
concentrations of urea above 4 M inactivate during incubation at 37°C for 5 min. 70% of the activity is recovered by dialysis | Vigna radiata var. radiata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Vigna radiata var. radiata | - |
seed | - |
Vicia faba | - |
seed | - |
Vicia sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.287 | - |
- |
Vicia sativa |
0.472 | - |
- |
Vicia faba |
0.566 | - |
- |
Vigna radiata var. radiata |
Storage Stability | Organism |
---|---|
-20°C, 40% w/v glycerol, 2 months with less than 10% loss of activity | Vigna radiata var. radiata |
4°C, 15% w/v glycerol, 48 h, stable | Vigna radiata var. radiata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-cyanoalanine + tRNAAsn | - |
Vigna radiata var. radiata | ? | - |
? | |
ATP + aspartate-3-hydroxamate + tRNAAsn | - |
Vigna radiata var. radiata | ? | - |
? | |
ATP + isoasparagine + tRNAAsn | - |
Vigna radiata var. radiata | ? | - |
? | |
ATP + L-asparagine + tRNAAsn | - |
Vigna radiata var. radiata | AMP + diphosphate + L-asparaginyl-tRNAAsn | - |
? | |
ATP + L-asparagine + tRNAAsn | - |
Vicia faba | AMP + diphosphate + L-asparaginyl-tRNAAsn | - |
? | |
ATP + L-asparagine + tRNAAsn | - |
Vicia sativa | AMP + diphosphate + L-asparaginyl-tRNAAsn | - |
? | |
additional information | ATP-diphosphate exchange | Vigna radiata var. radiata | ? | - |
? | |
additional information | acylate Phaseolus aureus tRNA with an efficiency of 68% compared to the Phaseolus aureus enzyme. The tRNA of Vicia faba and Vicia sativa appears to be completely interchangeable | Vicia faba | ? | - |
? | |
additional information | acylate Phaseolus aureus tRNA with an efficiency of 68% compared to the Phaseolus aureus enzyme. The tRNA of Vicia faba and Vicia sativa appears to be completely interchangeable | Vicia sativa | ? | - |
? | |
additional information | acylates tRNA of Vicia faba or Vicia sativa with an efficiency of 83% compared to Phaseolus aureus tRNA | Vigna radiata var. radiata | ? | - |
? | |
additional information | isoasparagine (with 96% of the efficiency compared to L-Asn), aspartate-3-hydroxamate (with 81% of the efficiency compared to L-Asn) and 3-cyanoalanine (with 36% of the efficiency compared to L-Asn) | Vigna radiata var. radiata | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
50% loss of activity after 5 min (without addition of stabilizer), after 14 min (with addition of 0.004 mM ATP), after 27 min (with addition of 0.05 mM Asn and ATP) | Vigna radiata var. radiata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
ATP-diphosphate exchange, tRNA esterification, Tris-chloride or Tris-acetate buffer | Vigna radiata var. radiata |