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Literature summary for 6.1.1.22 extracted from
- Amino acid substrate specificity of asparaginyl-, aspartyl- and glutaminyl-tRNA synthetase isolated from higher plants (1973), Phytochemistry, 12, 1903-1916.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ethanol | slight stimulation at low tRNA concentrations | Vigna radiata var. radiata |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-cyanoalanine | aminoacylation of Asn, competitive | Vigna radiata var. radiata | |
| Aspartate 3-hydroxamate | Asn-dependent ATP-diphosphate exchange, competitive | Vigna radiata var. radiata | |
| Br- | 1.2 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
| CH3COO- | 1.3 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
| Cl- | 2.5 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
| CNS- | 0.4 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
| Cs+ | 1.3 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
| I- | 0.5 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
| isoasparagine | aminoacylation of Asn, competitive | Vigna radiata var. radiata | |
| K+ | 2.5 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
| Li+ | 0.7 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
| Na+ | 2.0 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
| NH4+ | 0.9 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
| NO3- | 1.0 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
| p-chloromercuribenzoate | - |
Vigna radiata var. radiata | |
| threo-3-Hydroxyasparagine | aminoacylation of Asn, competitive | Vigna radiata var. radiata | |
| Tris | above 0.3 M | Vigna radiata var. radiata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
Asn | tRNA esterification | Vigna radiata var. radiata | |
| 0.18 | - |
Asn | ATP-diphosphate exchange | Vigna radiata var. radiata | |
| 0.26 | - |
isoasparagine | ATP-diphosphate exchange | Vigna radiata var. radiata | |
| 0.78 | - |
aspartate-3-hydroxamate | ATP-diphosphate exchange | Vigna radiata var. radiata | |
| 5.8 | - |
3-cyanoalanine | ATP-diphosphate exchange | Vigna radiata var. radiata | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates tRNA esterification with 5% of the efficiency compared to Mg2+ | Vigna radiata var. radiata | |
| Co2+ | stimulates ATP-diphosphate exchange with 17% the efficiency compared to Mg2+ and tRNA esterification with 45% | Vigna radiata var. radiata | |
| Mg2+ | required, optimal concentration is 20 mM in ATP-diphosphate exchange and 12 mM in tRNA esterification, Km: 5.8 mM in ATP-diphosphate exchange, 2.8 in tRNA esterification | Vigna radiata var. radiata | |
| Mn2+ | stimulates ATP-diphosphate exchange with 30% the efficiency compared to Mg2+ and tRNA esterification with 72% efficiency | Vigna radiata var. radiata | |
| Ni2+ | stimulates tRNA esterification with 15% of the efficiency compared to Mg2+ | Vigna radiata var. radiata | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vicia faba | - |
- |
- |
| Vicia sativa | - |
- |
- |
| Vigna radiata var. radiata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vicia faba |
- |
Vicia sativa |
- |
Vigna radiata var. radiata |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| concentrations of urea above 4 M inactivate during incubation at 37°C for 5 min. 70% of the activity is recovered by dialysis | Vigna radiata var. radiata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Vigna radiata var. radiata | - |
| seed | - |
Vicia faba | - |
| seed | - |
Vicia sativa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.287 | - |
- |
Vicia sativa |
| 0.472 | - |
- |
Vicia faba |
| 0.566 | - |
- |
Vigna radiata var. radiata |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 40% w/v glycerol, 2 months with less than 10% loss of activity | Vigna radiata var. radiata |
| 4°C, 15% w/v glycerol, 48 h, stable | Vigna radiata var. radiata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-cyanoalanine + tRNAAsn | - |
Vigna radiata var. radiata | ? | - |
? | |
| ATP + aspartate-3-hydroxamate + tRNAAsn | - |
Vigna radiata var. radiata | ? | - |
? | |
| ATP + isoasparagine + tRNAAsn | - |
Vigna radiata var. radiata | ? | - |
? | |
| ATP + L-asparagine + tRNAAsn | - |
Vigna radiata var. radiata | AMP + diphosphate + L-asparaginyl-tRNAAsn | - |
? | |
| ATP + L-asparagine + tRNAAsn | - |
Vicia faba | AMP + diphosphate + L-asparaginyl-tRNAAsn | - |
? | |
| ATP + L-asparagine + tRNAAsn | - |
Vicia sativa | AMP + diphosphate + L-asparaginyl-tRNAAsn | - |
? | |
| additional information | ATP-diphosphate exchange | Vigna radiata var. radiata | ? | - |
? | |
| additional information | acylate Phaseolus aureus tRNA with an efficiency of 68% compared to the Phaseolus aureus enzyme. The tRNA of Vicia faba and Vicia sativa appears to be completely interchangeable | Vicia faba | ? | - |
? | |
| additional information | acylate Phaseolus aureus tRNA with an efficiency of 68% compared to the Phaseolus aureus enzyme. The tRNA of Vicia faba and Vicia sativa appears to be completely interchangeable | Vicia sativa | ? | - |
? | |
| additional information | acylates tRNA of Vicia faba or Vicia sativa with an efficiency of 83% compared to Phaseolus aureus tRNA | Vigna radiata var. radiata | ? | - |
? | |
| additional information | isoasparagine (with 96% of the efficiency compared to L-Asn), aspartate-3-hydroxamate (with 81% of the efficiency compared to L-Asn) and 3-cyanoalanine (with 36% of the efficiency compared to L-Asn) | Vigna radiata var. radiata | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
50% loss of activity after 5 min (without addition of stabilizer), after 14 min (with addition of 0.004 mM ATP), after 27 min (with addition of 0.05 mM Asn and ATP) | Vigna radiata var. radiata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
ATP-diphosphate exchange, tRNA esterification, Tris-chloride or Tris-acetate buffer | Vigna radiata var. radiata |
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