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Literature summary for 6.1.1.21 extracted from

  • Tian, Q.; Wang, C.; Liu, Y.; Xie, W.
    Structural basis for recognition of G-1-containing tRNA by histidyl-tRNA synthetase (2015), Nucleic Acids Res., 43, 2980-2990 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme complexed with tRNAHis, L-histidine, and AMPPNP, hanging drop vapor diffusion method, using 5% (w/v) PEG 4000 and 0.1 M NaOAc (pH4.5) Thermus thermophilus

Protein Variants

Protein Variants Comment Organism
325-328Del the mutant shows about 20% of wild type activity Thermus thermophilus
328insTTET the mutant shows about 80% of wild type activity Thermus thermophilus
E114A inactive Thermus thermophilus
E388A the mutant shows about 15% of wild type activity Thermus thermophilus
E64A inactive Thermus thermophilus
G285A inactive Thermus thermophilus
G286A the mutant shows about 2% of wild type activity Thermus thermophilus
G72V the mutant shows about 50% of wild type activity Thermus thermophilus
G72V/G73V the mutant shows about 15% of wild type activity Thermus thermophilus
K209A the mutant shows about 80% of wild type activity Thermus thermophilus
K397Q the mutant shows about 10% of wild type activity Thermus thermophilus
Q404A the mutant shows about 90% of wild type activity Thermus thermophilus
R115A the mutant shows about 20% of wild type activity Thermus thermophilus
R120A the mutant shows about 8% of wild type activity Thermus thermophilus
R122A the mutant shows about 15% of wild type activity Thermus thermophilus
R122K the mutant shows about 55% of wild type activity Thermus thermophilus
R197A the mutant shows about 50% of wild type activity Thermus thermophilus
R7A the mutant shows about 20% of wild type activity Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-histidine + tRNAHis Thermus thermophilus
-
 AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus P62374
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-histidine + tRNAHis
-
 Thermus thermophilus AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Synonyms

Synonyms Comment Organism
HisRS
-
 Thermus thermophilus
Histidyl-tRNA synthetase
-
 Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Thermus thermophilus