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Literature summary for 6.1.1.21 extracted from
- Comparison of histidine recognition in human and trypanosomatid histidyl-tRNA synthetases (2014), Biochimie, 106, 111-120 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| vapor diffusion method, using 0.1 M Tris pH 8.5, 0.2 M MgCl2 and 25% (w/v) PEG 3350 | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
| cytosol | - |
Trypanosoma brucei | 5829 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-histidine + tRNAHis | Homo sapiens | - |
AMP + diphosphate + L-histidyl-tRNAHis | - |
? |  |
| ATP + L-histidine + tRNAHis | Trypanosoma brucei | - |
AMP + diphosphate + L-histidyl-tRNAHis | - |
? | |
| ATP + L-histidine + tRNAHis | Trypanosoma brucei CL Brener | - |
AMP + diphosphate + L-histidyl-tRNAHis | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P12081 | - |
- |
| Trypanosoma brucei | Q4DA54 | - |
- |
| Trypanosoma brucei CL Brener | Q4DA54 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, and Superdex 200 gel filtration | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-histidine + tRNAHis | - |
Homo sapiens | AMP + diphosphate + L-histidyl-tRNAHis | - |
? | |
| ATP + L-histidine + tRNAHis | - |
Trypanosoma brucei | AMP + diphosphate + L-histidyl-tRNAHis | - |
? | |
| ATP + L-histidine + tRNAHis | - |
Trypanosoma brucei CL Brener | AMP + diphosphate + L-histidyl-tRNAHis | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | - |
Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HisRS | - |
Homo sapiens |
| HisRS | - |
Trypanosoma brucei |
| Histidyl-tRNA synthetase | - |
Homo sapiens |
| Histidyl-tRNA synthetase | - |
Trypanosoma brucei |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
| ATP | - |
Trypanosoma brucei | |
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Release 2023.1 (January 2023)
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