Cloned (Comment) | Organism |
---|---|
expression of C-terminally epitope-tagged wild-type and mutant HARS2 in 293T cells mitochondria | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L200V | naturally occuring mutations in HARS2 involved in the Perrault syndrome, the mutant shows reduced activity compared to wild-type enzyme | Homo sapiens |
additional information | identification of a deletion mutant HARS2 DELTA200-211, probably involved in the Perrault syndrome, that is not stably expressed in mammalian mitochondria, its also shows no dimerization | Homo sapiens |
additional information | RNAi-dependent downregulation of hars-1, embryos from wild-type animals are laid onto bacteria expressing double-stranded RNA and the progeny are exposed to RNAi throughout development | Caenorhabditis elegans |
V368L | naturally occuring mutations in HARS2 involved in the Perrault syndrome, the mutant shows reduced activity compared to wild-type enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
mitochondrion | - |
Caenorhabditis elegans | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
Hars-1 is the single histidyl tRNA synthetase gene | - |
Homo sapiens | - |
- |
- |
Subunits | Comment | Organism |
---|---|---|
homodimer | HARS2 functions in vivo as a homodimer, HARS2 structure analysis, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
HARS1 | - |
Caenorhabditis elegans |
HARS2 | - |
Homo sapiens |
histidyl tRNA synthetase | - |
Homo sapiens |
histidyl tRNA synthetase | - |
Caenorhabditis elegans |
mitochondrial histidyl tRNA synthetase | - |
Homo sapiens |
mitochondrial histidyl tRNA synthetase | - |
Caenorhabditis elegans |
Organism | Comment | Expression |
---|---|---|
Caenorhabditis elegans | hars-1 RNAi-dependent downregulation of hars-1 decreases brood size in both wild-type and ced-4(n1162) mutants, but less severely in ced-4(n1162) mutants | down |
General Information | Comment | Organism |
---|---|---|
malfunction | the loss of fertility caused by hars-1 RNAi is partially mediated by apoptosis. Complete loss of hars-1 using the hars-1(tm4074) null allele. Homozygous hars-1 (tm4074) animals arrest development as L2 larvae, as determined by overall body size and by extent of gonad development, overview | Caenorhabditis elegans |
physiological function | hars-1 activity is required in Caenorhabditis elegans for fertility and proper germ cell development | Caenorhabditis elegans |