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Literature summary for 6.1.1.20 extracted from

  • Hu, Y.; Palmer, S.; Munoz, H.; Bullard, J.
    High throughput screen identifies natural product inhibitor of phenylalanyl-tRNA synthetase from Pseudomonas aeruginosa and Streptococcus pneumoniae (2015), Curr. Drug Discov. Technol., 11, 279-292 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2(DE3) cells Pseudomonas aeruginosa
expressed in Escherichia coli Rosetta 2(DE3) cells Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
1,1',6,6',7,7'-hexahydroxy-3,3'-dimethyl-5,5'-di(propan-2-yl)[2,2'-binaphthalene]-8,8'-dicarbaldehyde
-
 Pseudomonas aeruginosa
1,1',6,6',7,7'-hexahydroxy-3,3'-dimethyl-5,5'-di(propan-2-yl)[2,2'-binaphthalene]-8,8'-dicarbaldehyde
-
 Streptococcus pneumoniae
BPP_03B04
-
 Pseudomonas aeruginosa
BPP_03B04
-
 Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00094
-
 tRNAPhe at pH 7.5 and 37°C Streptococcus pneumoniae
0.0012
-
 tRNAPhe at pH 7.5 and 37°C Pseudomonas aeruginosa
0.021
-
 L-phenylalanine at pH 7.5 and 37°C Streptococcus pneumoniae
0.048
-
 L-phenylalanine at pH 7.5 and 37°C Pseudomonas aeruginosa
0.2
-
 ATP at pH 7.5 and 37°C Pseudomonas aeruginosa
0.225
-
 ATP at pH 7.5 and 37°C Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-phenylalanine + tRNAPhe Pseudomonas aeruginosa
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
ATP + L-phenylalanine + tRNAPhe Streptococcus pneumoniae
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Streptococcus pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Pseudomonas aeruginosa
Ni-NTA column chromatography Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-phenylalanine + tRNAPhe
-
 Pseudomonas aeruginosa AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
ATP + L-phenylalanine + tRNAPhe
-
 Streptococcus pneumoniae AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 80000 + 1 * 41000, SDS-PAGE Pseudomonas aeruginosa
heterodimer 1 * 85000 + 1 * 42000, SDS-PAGE Streptococcus pneumoniae

Synonyms

Synonyms Comment Organism
Phenylalanine-tRNA synthetase
-
 Pseudomonas aeruginosa
Phenylalanine-tRNA synthetase
-
 Streptococcus pneumoniae
PheRS
-
 Pseudomonas aeruginosa
PheRS
-
 Streptococcus pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.74
-
 tRNAPhe at pH 7.5 and 37°C Pseudomonas aeruginosa
0.93
-
 tRNAPhe at pH 7.5 and 37°C Streptococcus pneumoniae
13.2
-
 ATP at pH 7.5 and 37°C Pseudomonas aeruginosa
15.5
-
 ATP at pH 7.5 and 37°C Streptococcus pneumoniae
16.2
-
 L-phenylalanine at pH 7.5 and 37°C Pseudomonas aeruginosa
22.5
-
 L-phenylalanine at pH 7.5 and 37°C Streptococcus pneumoniae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Pseudomonas aeruginosa
ATP
-
 Streptococcus pneumoniae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00083
-
 BPP_03B04 at pH 7.5 and 37°C Pseudomonas aeruginosa
0.00083
-
 1,1',6,6',7,7'-hexahydroxy-3,3'-dimethyl-5,5'-di(propan-2-yl)[2,2'-binaphthalene]-8,8'-dicarbaldehyde at pH 7.5 and 37° Pseudomonas aeruginosa
0.00098
-
 BPP_03B04 at pH 7.5 and 37°C Streptococcus pneumoniae
0.00098
-
 1,1',6,6',7,7'-hexahydroxy-3,3'-dimethyl-5,5'-di(propan-2-yl)[2,2'-binaphthalene]-8,8'-dicarbaldehyde at pH 7.5 and 37° Streptococcus pneumoniae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0023
-
 at pH 7.5 and 37°C Pseudomonas aeruginosa BPP_03B04
0.0023
-
 at pH 7.5 and 37° Pseudomonas aeruginosa 1,1',6,6',7,7'-hexahydroxy-3,3'-dimethyl-5,5'-di(propan-2-yl)[2,2'-binaphthalene]-8,8'-dicarbaldehyde
0.0049
-
 at pH 7.5 and 37°C Streptococcus pneumoniae BPP_03B04
0.0049
-
 at pH 7.5 and 37° Streptococcus pneumoniae 1,1',6,6',7,7'-hexahydroxy-3,3'-dimethyl-5,5'-di(propan-2-yl)[2,2'-binaphthalene]-8,8'-dicarbaldehyde

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
64
-
 tRNAPhe at pH 7.5 and 37°C Pseudomonas aeruginosa
66
-
 ATP at pH 7.5 and 37°C Pseudomonas aeruginosa
70
-
 ATP at pH 7.5 and 37°C Streptococcus pneumoniae
340
-
 L-phenylalanine at pH 7.5 and 37°C Pseudomonas aeruginosa
1000
-
 tRNAPhe at pH 7.5 and 37°C Streptococcus pneumoniae
1100
-
 L-phenylalanine at pH 7.5 and 37°C Streptococcus pneumoniae