Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.20 extracted from

  • Banerjee, R.; Reynolds, N.M.; Yadavalli, S.S.; Rice, C.; Roy, H.; Banerjee, P.; Alexander, R.W.; Ibba, M.
    Mitochondrial aminoacyl-tRNA synthetase single-nucleotide polymorphisms that lead to defects in refolding but not aminoacylation (2011), J. Mol. Biol., 410, 280-293.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
N280S the mutant displays wild-type aminoacylation activity and stability with respect to their free energies of unfolding, but are less stable at low pH. It shows no significant loss in secondary structure. The mutant retains less activity than wild-type enzyme after refolding for mitochondrial import Homo sapiens
S57C the mutant displays wild-type aminoacylation activity and stability with respect to their free energies of unfolding, but are less stable at low pH. It shows no significant loss in secondary structure. The mutant retains less activity than wild-type enzyme after refolding for mitochondrial import Homo sapiens
S57C/N280S Ser57 and Asn280 map to positions away from the catalytic center and the anticodon binding domain of hmtPheRS, the mutant does not show significant loss in secondary structure or aminoacylation activity in vitro compared to wild-type enzyme. The S57C/N280S double mutant had remarkable stability even at low pH Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Subunits

Subunits Comment Organism
More structure molecular dynamics simulations of hmtPheRS, wild-type and mutant enzymes, overview Homo sapiens

Synonyms

Synonyms Comment Organism
mtPheRS
-
Homo sapiens
Phenylalanyl-tRNA synthetase
-
Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
1.5 8 high stability of wild-type and mutant mtPheRSs in the pH range, unfolding parameters, overview Homo sapiens