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Literature summary for 6.1.1.20 extracted from
- Expression and characterization of a human mitochondrial phenylalanyl-tRNA synthetase (1999), J. Mol. Biol., 288, 567-577.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mitochondrial isozyme, DNA sequence determination and analysis, expression in Escherichia coli BL21(DE3) as His-tagged enzyme | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
tRNAPhe | aminoacylation reaction, recombinant mitochondrial isozyme, pH 7.5, 37°C | Homo sapiens |  |
| 0.033 | - |
L-phenylalanine | ATP-diphosphate exchange reaction, recombinant mitochondrial isozyme, pH 7.3, 37°C | Homo sapiens | |
| 2.5 | - |
ATP | ATP-diphosphate exchange reaction, recombinant mitochondrial isozyme, pH 7.3, 37°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | mitochondrial isozyme | Homo sapiens | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | optimal at 5-10 mM, required, stimulates 2-4fold the second reaction step, the ester bond formation between the 2'-hydroxyl group of the 3'-terminal adenosine base of the tRNA and the carboxylic acid function of phenylalanine | Homo sapiens | |
| spermine | optimal at 1 mM, stimulates 2-4fold the second reaction step, the ester bond formation between the 2'-hydroxyl group of the 3'-terminal adenosine base of the tRNA and the carboxylic acid function of phenylalanine | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
recombinant mitochondrial isozyme, gel filtration | Homo sapiens |
| 48000 | - |
recombinant mitochondrial isozyme, analytical velocity sedimentation centrifugation | Homo sapiens |
| 49600 | - |
recombinant mitochondrial isozyme, amino acid sequence determination | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe | Homo sapiens | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O95363 | mitochondrial isozyme, class II enzyme | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe = AMP + diphosphate + L-phenylalanyl-tRNAPhe | two-step reaction, the second reaction step is the ester bond formation between the 2'-hydroxyl group of the 3'-terminal adenosine base of the tRNA and the carboxylic acid function of phenylalanine | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, purified recombinant His-tagged mitochondrial isozyme, 50 mM Tris-HCl, pH 7.6, 50 mM KCl, 10 mM MgCl2, 7 mM 2-mercaptoethanol, 10% v/v glycerol, small aliquots, stable for at least 6 months | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe | - |
Homo sapiens | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
ir | |
| ATP + L-phenylalanine + tRNAPhe | tRNAPhe substrate from Escherichia coli, two-step reaction, the first step, formation of the aminoacyl-adenylate, is reversible, the second, transfer of the activated amino acid to the tRNA, is not | Homo sapiens | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
ir | |
| additional information | the enzyme also performs the ATP-diphosphate exchange reaction | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 48000, recombinant mitochondrial isozyme, analytical sedimentation centrifugation | Homo sapiens |
| More | the mitochondrial isozyme is a single polypeptide chain, which bears similarities in structure to the alpha/beta subunit organization of bacterial enzymes | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CML33 | - |
Homo sapiens |
| FRS | - |
Homo sapiens |
| HSPC173 | - |
Homo sapiens |
| L-Phenylalanyl-tRNA synthetase | - |
Homo sapiens |
| mtPheRS | - |
Homo sapiens |
| Phenylalanine translase | - |
Homo sapiens |
| Phenylalanine--tRNA ligase | - |
Homo sapiens |
| Phenylalanine-tRNA synthetase | - |
Homo sapiens |
| Phenylalanyl transfer ribonucleic acid synthetase | - |
Homo sapiens |
| Phenylalanyl-transfer ribonucleate synthetase | - |
Homo sapiens |
| Phenylalanyl-transfer RNA ligase | - |
Homo sapiens |
| Phenylalanyl-transfer RNA synthetase | - |
Homo sapiens |
| Phenylalanyl-tRNA ligase | - |
Homo sapiens |
| Phenylalanyl-tRNA synthetase | - |
Homo sapiens |
| PheRS | - |
Homo sapiens |
| Synthetase, phenylalanyl-transfer ribonucleate | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
tRNAPhe | aminoacylation reaction, recombinant mitochondrial isozyme, pH 7.5, 37°C | Homo sapiens | |
| 1.1 | - |
L-phenylalanine | ATP-diphosphate exchange reaction, recombinant mitochondrial isozyme, pH 7.3, 37°C | Homo sapiens | |
| 2.9 | - |
ATP | ATP-diphosphate exchange reaction, mitochondrial isozyme, pH 7.3, 37°C | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
ATP-diphosphate exchnage assay at | Homo sapiens |
| 7.5 | - |
aminoacylation assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | mitochondrial isozyme, high concentration is required for maximal activity | Homo sapiens | |
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