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Literature summary for 6.1.1.20 extracted from

  • Moor, N.A.; Ankilova, V.N.; Lavrik, O.I.
    Recognition of tRNAPhe by phenylalanyl-tRNA synthetase of Thermus thermophilus (1995), Eur. J. Biochem., 234, 897-902.
    View publication on PubMed
Search for more literature for 6.1.1.20

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
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 additional information Km-value for tRNA mutants Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-phenylalanine + tRNAPhe mutant tRNAs with substitutions at position 16, 17, 19, or 20 (in the D loop), 34-36 (in the anticodon loop), 26, 44 (at the top of the anticodon stem), 56 (in the T loop), 73 (in the acceptor end) and at the base pairs 10*25 (in the D stem), 27*43 and 28*42 (in the anticodon). Nucleotide 20 and some tertiary nucleotides, including the conserved G19*C56 base pair, are proposed to participate in stabilization of the precise tRNA conformation required for efficient aminoacylation Thermus thermophilus AMP + diphosphate + L-phenylalanyl-tRNAPhe
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Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
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 Thermus thermophilus tRNAPhe, aminoacylation Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
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 additional information turnover numbers for tRNA mutants Thermus thermophilus