Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli B834 cells | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
in complex with L-tryptophan, hanging drop vapor diffusion method, using 0.03 M Na-NO3+ (0.03 M ammonium sulfate, 0.3 M Na2HPO4), 0.1 M bicin pH 8.5, 12.5% (w/v) 2-methyl-2,4-pentanediol, 12.5% (w/v) PEG 1000, 12.5% (w/v) PEG 3350, at 20°C | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Plasmodium falciparum | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73000 | - |
2 * 73000, SDS-PAGE | Plasmodium falciparum |
140000 | - |
gel filtration | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | Plasmodium falciparum | - |
AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8IDW3 | - |
- |
Purification (Comment) | Organism |
---|---|
heparin column chromatography and Superdex 200 gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | - |
Plasmodium falciparum | AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 73000, SDS-PAGE | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
Tryptophanyl-tRNA synthetase | - |
Plasmodium falciparum |
WRS | - |
Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Plasmodium falciparum |