Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.2 extracted from

  • Weinreb, V.; Li, L.; Chandrasekaran, S.N.; Koehl, P.; Delarue, M.; Carter, C.W.
    Enhanced amino acid selection in fully evolved tryptophanyl-tRNA synthetase, relative to its urzyme, requires domain motion sensed by the D1 switch, a remote dynamic packing motif (2014), J. Biol. Chem., 289, 4367-4376.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000943
-
 L-tryptophan wild type enzyme, in 0.1 M Tris-HCl (pH 7.5), 0.01 M potassium fluoride, 5 mM MgCl2, at 37°C Geobacillus stearothermophilus
0.00527
-
 L-tryptophan wild type enzyme, in 0.1 M Tris-HCl (pH 7.5), 0.01 M potassium fluoride, 5 mM MnCl2, at 37°C Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Geobacillus stearothermophilus
Mn2+ replacing Mg2+ with Mn2+ reduces the specificity for L-tryptophan, increasing the relative activation of L-tyrosine by about 3.5fold Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-tryptophan + tRNATrp Geobacillus stearothermophilus
-
 AMP + diphosphate + L-tryptophyl-tRNATrp
-
 ?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus P00953
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-tryptophan + tRNATrp
-
 Geobacillus stearothermophilus AMP + diphosphate + L-tryptophyl-tRNATrp
-
 ?
additional information the enzyme also accepts L-tyrosine as substrate, albeit with lower catalytic efficiency Geobacillus stearothermophilus ?
-
 ?

Synonyms

Synonyms Comment Organism
TrpRS
-
 Geobacillus stearothermophilus
Tryptophanyl-tRNA synthetase
-
 Geobacillus stearothermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.88
-
 L-tryptophan wild type enzyme, in 0.1 M Tris-HCl (pH 7.5), 0.01 M potassium fluoride, 5 mM MnCl2, at 37°C Geobacillus stearothermophilus
5.45
-
 L-tryptophan wild type enzyme, in 0.1 M Tris-HCl (pH 7.5), 0.01 M potassium fluoride, 5 mM MgCl2, at 37°C Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Geobacillus stearothermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
164
-
 L-tryptophan wild type enzyme, in 0.1 M Tris-HCl (pH 7.5), 0.01 M potassium fluoride, 5 mM MnCl2, at 37°C Geobacillus stearothermophilus
1090
-
 L-tryptophan wild type enzyme, in 0.1 M Tris-HCl (pH 7.5), 0.01 M potassium fluoride, 5 mM MgCl2, at 37°C Geobacillus stearothermophilus