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Literature summary for 6.1.1.2 extracted from
- Species-specific differences in the regulation of the aminoacylation activity of mammalian tryptophanyl-tRNA synthetases (2010), FEBS Lett., 584, 229-232.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Homo sapiens |  |
| heme | R135H/E438A double mutant | Bos taurus | |
| Zn2+ | - |
Homo sapiens | |
| Zn2+ | R135H/E438A double mutant | Bos taurus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pET20b for expression in Escherichia coli BL21DE3 cells | Mus musculus |
| into the vector pET20b for expression in Escherichia coli BL21DE3 cells | Bos taurus |
| into the vector pET20b for expression in Escherichia coli BL21DE3 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E438A | TrpRS single mutant | Bos taurus |
| H130R | mutant, constitutively active | Homo sapiens |
| R135H | TrpRS single mutant | Bos taurus |
| R135H/E438A | TrpRS double mutant, binds with Zn2+ or heme to enhance its aminoacylation activity | Bos taurus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | - |
Bos taurus | |
| Zn2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-tryptophan + tRNATrp | Mus musculus | - |
AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? | |
| ATP + L-tryptophan + tRNATrp | Bos taurus | - |
AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? | |
| ATP + L-tryptophan + tRNATrp | Homo sapiens | - |
AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Homo sapiens | P23381 | - |
- |
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| on a nickel affinity column | Mus musculus |
| on a nickel affinity column | Bos taurus |
| on a nickel affinity column | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-tryptophan + tRNATrp | - |
Mus musculus | AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? | |
| ATP + L-tryptophan + tRNATrp | - |
Bos taurus | AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? | |
| ATP + L-tryptophan + tRNATrp | - |
Homo sapiens | AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TrpRS | - |
Mus musculus |
| TrpRS | - |
Bos taurus |
| TrpRS | - |
Homo sapiens |
| Tryptophanyl-tRNA synthetase | - |
Mus musculus |
| Tryptophanyl-tRNA synthetase | - |
Bos taurus |
| Tryptophanyl-tRNA synthetase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
aminoacylation assay | Mus musculus |
| 20 | - |
aminoacylation assay | Bos taurus |
| 20 | - |
aminoacylation assay | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
aminoacylation assay | Mus musculus |
| 7.5 | - |
aminoacylation assay | Bos taurus |
| 7.5 | - |
aminoacylation assay | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Mus musculus | |
| ATP | - |
Bos taurus | |
| ATP | - |
Homo sapiens | |
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