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Literature summary for 6.1.1.2 extracted from
- Three G.C base pairs required for the efficient aminoacylation of tRNATrp by tryptophanyl-tRNA synthetase from Bacillus subtilis (2002), Biochemistry, 41, 8087-8092.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of tRNATrp mutants altered in the acceptor stem region, overview | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic investigation of enzyme activity with diverse tRNATrp mutants | Bacillus subtilis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Bacillus subtilis |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-tryptophan + tRNATrp | Bacillus subtilis | - |
AMP + diphosphate + L-tryptophan-tRNATrp | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
purified homogenic recombinant wild-type enzyme | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-tryptophan + tRNATrp | - |
Bacillus subtilis | AMP + diphosphate + L-tryptophan-tRNATrp | - |
? | |
| ATP + L-tryptophan + tRNATrp | the 3 base pairs G2.C71, G3.C70, and G4.C69 are important identity elements in the tRNA acceptor stem providing tRNA substrate recognition for the enzyme, also the G73 discriminator base is involved in the recognition but cannot itself confer efficient aminoacylation activity | Bacillus subtilis | AMP + diphosphate + L-tryptophan-tRNATrp | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TrpRS | - |
Bacillus subtilis |
| Tryptophanyl-tRNA synthase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bacillus subtilis | |
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Release 2023.1 (January 2023)
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