Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
DELTANhcArgRS | N-terminal 72-amino acid deletion mutant with higher specific activity than the wild type enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
SDS-PAGE, mutant enzyme DELTANhcArgRS | Homo sapiens |
75000 | - |
SDS-PAGE, wild type enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA Superflow resin chromatography and dialysis against 20 mM potassium phosphate buffer (pH 7.5) | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryonic fibroblast | - |
Homo sapiens | - |
HEK-293 cell | - |
Homo sapiens | - |
Hep-G2 cell | - |
Homo sapiens | - |
hepatoma cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.286 | - |
wild type enzyme, in 50 mM Tris-HCl (pH 7.5) | Homo sapiens |
0.715 | - |
mutant enzyme DELTANhcArgRS, in 50 mM Tris-HCl (pH 7.5) | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | - |
Homo sapiens | AMP + diphosphate + L-arginyl-tRNAArg | - |
? |
Synonyms | Comment | Organism |
---|---|---|
arginyltRNA synthetase | - |
Homo sapiens |
ArgRS | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
tRNAArg | wild type enzyme, in 50 mM Tris-HCl (pH 7.5) | Homo sapiens | |
0.8 | - |
tRNAArg | mutant enzyme DELTANhcArgRS, in 50 mM Tris-HCl (pH 7.5) | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |