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Literature summary for 6.1.1.19 extracted from

  • Kiga, D.; Sakamoto, K.; Sato, S.; Hirao, I.; Yokoyama, S.
    Shifted positioning of the anticodon nucleotide residues of amber suppressor tRNA species by Escherichia coli arginyl-tRNA synthetase (2001), Eur. J. Biochem., 268, 6207-6213.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the wild type and the argS1 mutant enzymes in Escherichia coli Escherichia coli

Protein Variants

Protein Variants Comment Organism
M460V mutant cannot suppress the effect of the expression of FTOR126, a variant of the wild type tRNAArg Escherichia coli
Y524D mutant cannot suppress the effect of the expression of FTOR126, a variant of the wild type tRNAArg Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information effect on the Km of several variations of the tRNAArg sequence Escherichia coli
0.00044
-
tRNAArg argS1 mutant, pH 7.5, 37°C Escherichia coli
0.001
-
tRNAArg pH 7.5, 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using chromatography on Ni-nitrilotriacetic acid agarose Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
recombinant enzyme tagged with 10 tandem histidine residues at the N-terminus exhibits similar activity than the wild type enzyme, C-terminal tag inactivates the enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-arginine + tRNAArg cytidine in position 35 and G or U in position 36 of the tRNAArg are required for ArgRS activity in Escherichia coli Escherichia coli AMP + diphosphate + L-arginyl-tRNAArg
-
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Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information effect on the turnover number of several variations of the tRNAArg sequence Escherichia coli
2.8
-
tRNAArg argS1 mutant, pH 7.5, 37°C Escherichia coli
17
-
tRNAArg pH 7.5, 37°C Escherichia coli