Cloned (Comment) | Organism |
---|---|
overexpression of the wild type and the argS1 mutant enzymes in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
M460V | mutant cannot suppress the effect of the expression of FTOR126, a variant of the wild type tRNAArg | Escherichia coli |
Y524D | mutant cannot suppress the effect of the expression of FTOR126, a variant of the wild type tRNAArg | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | effect on the Km of several variations of the tRNAArg sequence | Escherichia coli | |
0.00044 | - |
tRNAArg | argS1 mutant, pH 7.5, 37°C | Escherichia coli | |
0.001 | - |
tRNAArg | pH 7.5, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using chromatography on Ni-nitrilotriacetic acid agarose | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant enzyme tagged with 10 tandem histidine residues at the N-terminus exhibits similar activity than the wild type enzyme, C-terminal tag inactivates the enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | cytidine in position 35 and G or U in position 36 of the tRNAArg are required for ArgRS activity in Escherichia coli | Escherichia coli | AMP + diphosphate + L-arginyl-tRNAArg | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | effect on the turnover number of several variations of the tRNAArg sequence | Escherichia coli | |
2.8 | - |
tRNAArg | argS1 mutant, pH 7.5, 37°C | Escherichia coli | |
17 | - |
tRNAArg | pH 7.5, 37°C | Escherichia coli |