Protein Variants | Comment | Organism |
---|---|---|
W162A | expression in Escherichia coli, 23% of wild-type activity | Escherichia coli |
W172A | expression in Escherichia coli | Escherichia coli |
W228A | expression in Escherichia coli | Escherichia coli |
W349A | expression in Escherichia coli | Escherichia coli |
W446A | expression in Escherichia coli | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
tRNAArg | mutant W162A, pH 7.4, 37ºC | Escherichia coli | |
0.0025 | - |
tRNAArg | pH 7.4, 37ºC | Escherichia coli | |
0.0073 | - |
L-arginine | mutant W162A, pH 7.4, 37ºC | Escherichia coli | |
0.012 | - |
L-arginine | pH 7.4, 37ºC | Escherichia coli | |
0.84 | - |
ATP | mutant W162A, pH 7.4, 37ºC | Escherichia coli | |
0.9 | - |
ATP | pH 7.4, 37ºC | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification of recombinant enzymes using DEAE-Sepharose and Blue-Sepharose column chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
mutant W162A displays 23% of the activity compared to the native enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | - |
Escherichia coli | AMP + diphosphate + L-arginyl-tRNAArg | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.6 | - |
ATP | mutant W162A, pH 7.4, 37ºC | Escherichia coli | |
26 | - |
ATP | pH 7.4, 37ºC | Escherichia coli |