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Literature summary for 6.1.1.19 extracted from

  • Eriani, G.; Dirheimer, G.; Gangloff, J.
    Structure-function relationship of arginyl-tRNA synthetase from Escherichia coli: isolation and characterization of the argS mutation MA5002 (1990), Nucleic Acids Res., 18, 1475-1479.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Protein Variants

Protein Variants Comment Organism
argS MA5002 mutant argS MA5002 differs from the wild-type ArgRS structural gene by one mutation, a substitution of an Arg by a Ser at position 134. It exhibits a 4times to 6times as low activity and a 5times as high Km value for ATP as the wild-type enzyme in aminoacylation and ATP-diphosphate exchange, Km values for Arg and tRNAArg remain unaltered Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
tRNAArg aminoacylation, native enzyme Escherichia coli
0.0042
-
tRNAArg aminoacylation, mutant enzyme Escherichia coli
0.009
-
Arg aminoacylation, native enzyme Escherichia coli
0.011
-
Arg aminoacylation and ATP-diphosphate exchange, mutant enzyme Escherichia coli
0.11
-
Arg ATP-diphosphate exchange Escherichia coli
0.15
-
Arg ATP-diphosphate exchange, native enzyme Escherichia coli
0.7
-
Arg aminoacylation, native enzyme Escherichia coli
2
-
ATP ATP-diphosphate exchange, native enzyme Escherichia coli
4.3
-
ATP aminoacylation, mutant enzyme Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli
-
mutant argS MA5002
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-arginine + tRNAArg
-
Escherichia coli AMP + diphosphate + L-arginyl-tRNAArg
-
?
additional information arginine-dependent ATP-diphosphate exchange Escherichia coli ?
-
?