Literature summary for 6.1.1.18 extracted from

Gruic-Sovulj, I.; Uter, N.; Bullock, T.; Perona, J.J.
tRNA-dependent aminoacyl-adenylate hydrolysis by a nonediting class I aminoacyl-tRNA synthetase (2005), J. Biol. Chem., 280, 23978-23986.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of the GlnRS-tRNA(2'H)Gln complex bound to the ATP analog AMPCPP and glutamine are grown by microseeding with crystals of the GlnRS-tRNAGln-ATP ternary complex. Crystals grew in 1-2 weeks by vapor diffusion over a reservoir containing 2 M ammonium sulfate, 10 mM Pipes, pH 7.5, 10 mM MgCl2 and 2 mM DTT	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
10.4	-	L-glutamine	reaction with tRNA(2'H)Gln	Escherichia coli
660	-	ATP	reaction with tRNA(2'H)Gln	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-glutamine + tRNAGln	-	Escherichia coli	AMP + diphosphate + L-glutaminyl-tRNAGln	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.04	-	L-glutamine	reaction with tRNA(2'H)Gln	Escherichia coli

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Release 2023.1 (January 2023)