Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme in complex with tRNAGlu, hanging-drop vapour diffusion method, precipitant solution contains 37 mM Na-MOPS, pH 6.7, 22% PEG 1500, 37 mM ammonium sulfate, 1% 2-methyl-2,4-pentanediol, 10 mM MgCl2, 5 mM 2-mercaptoethanol, X-ray diffraction structure determination at 2.4 A resolution, and analysis | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
R358Q | site-directed mutagenesis, exchange of the Arg residue results in a mutant that no longer discriminates between tRNAGlu and tRNAGln anticodons YUC and YUG, respectively | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0047 | - |
wild type tRNAGlu | recombinant wild-type enzyme, 65°C | Thermus thermophilus | |
0.043 | - |
tRNAGlu mutant C36G | recombinant wild-type enzyme, 65°C | Thermus thermophilus | |
0.055 | - |
tRNAGlu mutant C36G | recombinant mutant R358Q, 65°C | Thermus thermophilus | |
0.085 | - |
wild type tRNAGlu | recombinant mutant R358Q, 65°C | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGlu | Thermus thermophilus | - |
AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | P27000 | discriminating enzyme form, purified recombinant enzyme from overexpressing Escherichia coli strain JM109(DE3) | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGlu | - |
Thermus thermophilus | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
ATP + L-glutamate + tRNAGlu mutant C36G | mutant R358Q, low activity with the wild-type enzyme | Thermus thermophilus | AMP + diphosphate + L-glutamyl-tRNAGlu mutant C36G | - |
? | |
ATP + L-glutamate + tRNAGlu wild-type | enzyme is specific for tRNAGlu | Thermus thermophilus | AMP + diphosphate + L-glutamyl-tRNAGlu wild-type | - |
? | |
ATP + L-glutamate + wild type tRNAGlu | - |
Thermus thermophilus | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GluRS | - |
Thermus thermophilus |
Glutamyl-tRNA synthetase | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
tRNAGlu mutant C36G | recombinant wild-type enzyme, 65°C | Thermus thermophilus | |
1.4 | - |
tRNAGlu mutant C36G | recombinant mutant enzyme R358Q, 65°C | Thermus thermophilus | |
1.5 | - |
wild type tRNAGlu | recombinant mutant enzyme R358Q, 65°C | Thermus thermophilus | |
2.1 | - |
wild type tRNAGlu | recombinant wild-type enzyme, 65°C | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermus thermophilus |