Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGlu | - |
Escherichia coli | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
aminoacylation | Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 55 | 35°C: about 70% of maximal activity, 55°C: about 60% of maximal activity | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
tRNAGlu and ATP protect efficiently against thermal inactivation, glutamate does not. Weak synergism between ATP and tRNAGlu, no synergism between ATP and glutamate. Highest stabilization with ATP, glutamate and tRNAGlu | Escherichia coli |
42 | - |
stable up to, without addition of substrate | Escherichia coli |
50 | - |
50% loss of activity, when temperature is increased gradually at the rate of 1 C per min, without addition of substrate | Escherichia coli |
60 | - |
complete loss of activity, when temperature is increased gradually at the rate of 1°C per min, without addition of substrate | Escherichia coli |