Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, crystals of enzyme bound to tRNACys at a resolution of 2.3 A | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
tRNACys | mutant enzyme E354A, wild-type tRNACys(G15-G48) | Escherichia coli | |
0.00058 | - |
tRNACys | mutant enzyme E354Q, wild-type tRNACys(G15-G48) | Escherichia coli | |
0.00116 | - |
tRNACys | wild-type enzyme, wild-type tRNACys(G15-G48) | Escherichia coli | |
0.0088 | - |
tRNACys | mutant enzyme N351A, wild-type tRNACys(G15-G48) | Escherichia coli | |
0.0177 | - |
tRNACys | mutant enzyme N351D, mutant tRNACys(G15-C48) | Escherichia coli | |
0.0179 | - |
tRNACys | mutant enzyme E354Q, wild-type tRNACys(G15-G48) | Escherichia coli | |
0.0185 | - |
tRNACys | mutant enzyme N351D, wild-type tRNACys(G15-G48) | Escherichia coli | |
0.0332 | - |
tRNACys | mutant enzyme N351A, mutant tRNACys(G15-C48) | Escherichia coli | |
0.0358 | - |
tRNACys | wild-type enzyme, mutant tRNACys(G15-C48) | Escherichia coli | |
0.0435 | - |
tRNACys | mutant enzyme E354A, mutant tRNACys(G15-C48) | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P21888 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-cysteine + tRNACys | - |
Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
tRNACys | mutant enzyme N351D, mutant tRNACys(G15-C48) | Escherichia coli | |
0.036 | - |
tRNACys | mutant enzyme E354A, mutant tRNACys(G15-C48) | Escherichia coli | |
0.074 | - |
tRNACys | mutant enzyme E354Q, mutant tRNACys(G15-C48) | Escherichia coli | |
0.094 | - |
tRNACys | mutant enzyme N351D, wild-type tRNACys(G15-G48) | Escherichia coli | |
0.1 | - |
tRNACys | mutant enzyme E354A, wild-type tRNACys(G15-G48) | Escherichia coli | |
0.14 | - |
tRNACys | mutant enzyme E354Q, wild-type tRNACys(G15-G48) | Escherichia coli | |
0.39 | - |
tRNACys | mutant enzyme N351A, mutant tRNACys(G15-C48) | Escherichia coli | |
0.45 | - |
tRNACys | wild-type enzyme, mutant tRNACys(G15-C48) | Escherichia coli | |
0.59 | - |
tRNACys | mutant enzyme N351A, wild-type tRNACys(G15-G48) | Escherichia coli | |
2.46 | - |
tRNACys | wild-type enzyme, wild-type tRNACys(G15-G48) | Escherichia coli |