Literature summary for 6.1.1.16 extracted from

Newberry, K.J.; Hou, Y.M.; Perona, J.J.
Structural origins of amino acid selection without editing by cysteinyl-tRNA synthetase (2002), EMBO J., 21, 2778-2787.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant free enzyme or cysteine and ATP complexed enzyme, hanging-drop vapour diffusion method, 17°C, 0.001 ml protein solution: 6 mg/ml enzyme, 10 mM HEPES, pH 7.4, 50 mM NaCl, 1 mM DTT, 5 mM MgCl2, 5 mM ATP, 10 mM cysteine, plus equal volume of reservoir solution: 0.1 M sodium cacodylate, pH 6.5, 15-17% PEG 8000, 0.2 M magnesium acetate, 2% tert-butanol, 1-3 weeks, cysteine but not ATP is required for crystal growth, X-ray diffraction structure determination at 2.3-3.0 A resolution, and analysis	Escherichia coli

Crystallization (Comment)

Organism

purified recombinant free enzyme or cysteine and ATP complexed enzyme, hanging-drop vapour diffusion method, 17°C, 0.001 ml protein solution: 6 mg/ml enzyme, 10 mM HEPES, pH 7.4, 50 mM NaCl, 1 mM DTT, 5 mM MgCl2, 5 mM ATP, 10 mM cysteine, plus equal volume of reservoir solution: 0.1 M sodium cacodylate, pH 6.5, 15-17% PEG 8000, 0.2 M magnesium acetate, 2% tert-butanol, 1-3 weeks, cysteine but not ATP is required for crystal growth, X-ray diffraction structure determination at 2.3-3.0 A resolution, and analysis

Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	1 ion bound at the base of the active site cleft, important for tRNA substrate specificity, single direct interaction with cysteine thiolate substrate	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-cysteine + tRNACys	Escherichia coli	-	AMP + diphosphate + L-cysteinyl-tRNACys	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P21888	class I enzyme	-

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + L-cysteine + tRNACys = AMP + diphosphate + L-cysteinyl-tRNACys	substrate binding mechanism, induced fit	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-cysteine + tRNACys	-	Escherichia coli	AMP + diphosphate + L-cysteinyl-tRNACys	-	?
ATP + L-cysteine + tRNACys	enzyme is highly specific for L-cysteine and does not possess the editing activity characteristic for other tRNA synthetases	Escherichia coli	AMP + diphosphate + L-cysteinyl-tRNACys	-	?

Subunits

Subunits	Comment	Organism
monomer	-	Escherichia coli

Synonyms

Synonyms	Comment	Organism
CysRS	-	Escherichia coli
Cysteine translase	-	Escherichia coli
Cysteine--tRNA ligase	-	Escherichia coli
Cysteinyl-transfer ribonucleate synthetase	-	Escherichia coli
Cysteinyl-transfer RNA synthetase	-	Escherichia coli
Cysteinyl-tRNA synthetase	-	Escherichia coli
Synthetase, cysteinyl-transfer ribonucleate	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Escherichia coli