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Literature summary for 6.1.1.16 extracted from

  • Burnell, J.N.; Whatley, F.R.
    Sulphur metabolism in Paracoccus denitrificans. Purification, properties and regulation of cysteinyl- and methionyl-tRNA synthetase (1977), Biochim. Biophys. Acta, 481, 266-278.
    View publication on PubMed
Search for more literature for 6.1.1.16

Activating Compound

Activating Compound Comment Organism Structure
methionine stimulates Paracoccus denitrificans

Inhibitors

Inhibitors Comment Organism Structure
2-L-aminobutyric acid
-
 Paracoccus denitrificans
3'-AMP
-
 Paracoccus denitrificans
5'-AMP
-
 Paracoccus denitrificans
ADP
-
 Paracoccus denitrificans
ATP free ATP inhibits Paracoccus denitrificans
cysteamine
-
 Paracoccus denitrificans
D-cysteine
-
 Paracoccus denitrificans
EDTA
-
 Paracoccus denitrificans
glutathione
-
 Paracoccus denitrificans
iodoacetamide
-
 Paracoccus denitrificans
L-alanine
-
 Paracoccus denitrificans
L-cysteic acid
-
 Paracoccus denitrificans
L-homocysteine
-
 Paracoccus denitrificans
L-selenocysteine
-
 Paracoccus denitrificans
Mg2+ free Mg2+ inhibits Paracoccus denitrificans
NaF weak Paracoccus denitrificans
NEM
-
 Paracoccus denitrificans
O-acetylserine
-
 Paracoccus denitrificans
p-chloromercuribenzoate
-
 Paracoccus denitrificans
phenylhydrazine weak Paracoccus denitrificans
phosphate
-
 Paracoccus denitrificans
S2-
-
 Paracoccus denitrificans
Sulfhydryl group reagents
-
 Paracoccus denitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00159
-
 selenocysteine ATP-diphosphate exchange Paracoccus denitrificans
0.0125
-
 L-Cys ATP-diphosphate exchange Paracoccus denitrificans
0.3
-
 diphosphate ATP-diphosphate exchange Paracoccus denitrificans
1
-
 diphosphate ATP-diphosphate exchange Paracoccus denitrificans
1.33
-
 ATP ATP-diphosphate exchange Paracoccus denitrificans
10
-
 2-aminobutyric acid ATP-diphosphate exchange Paracoccus denitrificans

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 13% of the efficiency of Mg2+ in activation of ATP-diphosphate exchange Paracoccus denitrificans
Mg2+ required for ATP-diphosphate exchange Paracoccus denitrificans
Mg2+ inhibited by free Mg2+ Paracoccus denitrificans
Mg2+ optimal Mg2+/ATP ratio is 2.5 Paracoccus denitrificans
Zn2+ 6% of the efficiency of Mg2+ in activation of ATP-diphosphate exchange Paracoccus denitrificans

Organism

Organism UniProt Comment Textmining
Paracoccus denitrificans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Paracoccus denitrificans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Paracoccus denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-aminobutyric acid + tRNACys
-
 Paracoccus denitrificans AMP + diphosphate + L-alpha-aminobutyryl-tRNACys
-
 ?
ATP + L-cysteine + tRNACys
-
 Paracoccus denitrificans AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?
ATP + L-cysteine + tRNACys
-
 Paracoccus denitrificans 8944 AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?
ATP + selenocysteine + tRNACys
-
 Paracoccus denitrificans AMP + diphosphate + selenocysteinyl-tRNACys
-
 ?
additional information ATP-diphosphate exchange Paracoccus denitrificans ?
-
 ?
additional information selenocysteine can replace cysteine in ATP-diphosphate exchange Paracoccus denitrificans ?
-
 ?
additional information 2-aminobutyric acid can replace cysteine in ATP-diphosphate exchange Paracoccus denitrificans ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
 Paracoccus denitrificans