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Literature summary for 6.1.1.15 extracted from
- Inhibition of Arabidopsis growth by the allelopathic compound azetidine-2-carboxylate is due to the low amino acid specificity of cytosolic prolyl-tRNA synthetase (2016), Plant J., 88, 236-246 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21-Codon Plus (DE3) cells | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azetidine-2-carboxylic acid | at 0.2 mM isozyme ProRS-Org is inhibited by 25% | Arabidopsis thaliana |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
L-proline | isozyme ProRS-Cyt, at pH 7.2 and 30°C | Arabidopsis thaliana | |
| 0.1 | - |
L-proline | isozyme ProRS-Org, at pH 7.2 and 30°C | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | isozyme ProRS-Org | Arabidopsis thaliana | 9507 | - |
| cytosol | isozyme ProRS-Cyt | Arabidopsis thaliana | 5829 | - |
| mitochondrion | isozyme ProRS-Org | Arabidopsis thaliana | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-proline + tRNAPro | Arabidopsis thaliana | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| callus | - |
Arabidopsis thaliana | - |
| cotyledon | - |
Arabidopsis thaliana | - |
| root | - |
Arabidopsis thaliana | - |
| seedling | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-proline + tRNAPro | - |
Arabidopsis thaliana | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Prolyl-tRNA synthetase | - |
Arabidopsis thaliana |
| ProRS-Cyt | isozyme | Arabidopsis thaliana |
| ProRS-Org | isozyme | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 13.2 | - |
L-proline | isozyme ProRS-Org, at pH 7.2 and 30°C | Arabidopsis thaliana | |
| 57.5 | - |
L-proline | isozyme ProRS-Cyt, at pH 7.2 and 30°C | Arabidopsis thaliana | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Arabidopsis thaliana | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
isozyme ProRS-Org, at pH 7.2 and 30°C | Arabidopsis thaliana | azetidine-2-carboxylic acid | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 132 | - |
L-proline | isozyme ProRS-Org, at pH 7.2 and 30°C | Arabidopsis thaliana | |
| 958 | - |
L-proline | isozyme ProRS-Cyt, at pH 7.2 and 30°C | Arabidopsis thaliana | |
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