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Literature summary for 6.1.1.15 extracted from

  • Norris, R.D.; Fowden, L.
    Substrate discrimination by prolyl-tRNA synthetase from various higher plants (1972), Phytochemistry, 11, 2921-2935.
No PubMed abstract available
Search for more literature for 6.1.1.15

General Stability

General Stability Organism
L-azetidine-2-carboxylic acid, cis(exo)-3,4-methano-L-proline, 3,4-dehydro-DL-proline, L-thiazolidine-4-carboxylic acid, N-methylglycine, N-methyl-L-alanine or trans-3-hydroxy-L-proline protect against inactivation at 70°C and 40°C Phaseolus vulgaris
L-azetidine-2-carboxylic acid, cis(exo)-3,4-methano-L-proline, 3,4-dehydro-DL-proline, L-thiazolidine-4-carboxylic acid, N-methylglycine, N-methyl-L-alanine or trans-3-hydroxy-L-proline protect against inactivation at 70°C and 40°C Delonix regia
Pro or ATP markedly stabilizes against heat inactivation Phaseolus vulgaris
Pro or ATP markedly stabilizes against heat inactivation Delonix regia
Pro or ATP markedly stabilizes against heat inactivation Parkinsonia aculeata
several analogues of Pro protect against thermal inactivation Phaseolus vulgaris
several analogues of Pro protect against thermal inactivation Delonix regia

Inhibitors

Inhibitors Comment Organism Structure
2-Pyrrolidinone
-
 Phaseolus vulgaris
3,4-Dehydroproline
-
 Delonix regia
3,4-Dehydroproline
-
 Phaseolus vulgaris
3-pyrroline
-
 Delonix regia
3-pyrroline
-
 Phaseolus vulgaris
4-Methylene-DL-proline weak Delonix regia
cis(exo)-3,4-Methano-L-proline
-
 Delonix regia
cis(exo)-3,4-Methano-L-proline
-
 Phaseolus vulgaris
cis-3-hydroxy-L-proline weak Delonix regia
cis-3-hydroxy-L-proline weak Phaseolus vulgaris
CsCl concentration required to inhibit ATP-diphosphate exchange by 50%: above 2 M Beta vulgaris
CsCl concentration required to inhibit ATP-diphosphate exchange by 50%: 0.5 M Delonix regia
CsCl concentration required to inhibit ATP-diphosphate exchange by 50%: 0.51 M Parkinsonia aculeata
CsCl concentration required to inhibit ATP-diphosphate exchange by 50%: 1.7 M Phaseolus vulgaris
DL-Proline amide
-
 Phaseolus vulgaris
KCl concentration required to inhibit ATP-diphosphate exchange by 50%: above 2 M Beta vulgaris
KCl concentration required to inhibit ATP-diphosphate exchange by 50%: 0.37 M Delonix regia
KCl concentration required to inhibit ATP-diphosphate exchange by 50%: 0.4 M Parkinsonia aculeata
KCl concentration required to inhibit ATP-diphosphate exchange by 50%: 2.1 M Phaseolus vulgaris
L-azetidine-2-carboxylic acid
-
 Delonix regia
L-azetidine-2-carboxylic acid
-
 Phaseolus vulgaris
L-thiazolidine-4-carboxylic acid
-
 Delonix regia
L-thiazolidine-4-carboxylic acid
-
 Phaseolus vulgaris
LiCl concentration required to inhibit ATP-diphosphate exchange by 50%: 1.2 M Beta vulgaris
LiCl concentration required to inhibit ATP-diphosphate exchange by 50%: 0.25 M Delonix regia
LiCl concentration required to inhibit ATP-diphosphate exchange by 50%: 0.23 M Parkinsonia aculeata
LiCl concentration required to inhibit ATP-diphosphate exchange by 50%: 1.2 M Phaseolus vulgaris
N-ethylglycine weak Delonix regia
N-Methyl-L-alanine weak Phaseolus vulgaris
N-methylglycine weak Delonix regia
N-methylglycine
-
 Phaseolus vulgaris
NaCl concentration required to inhibit ATP-diphosphate exchange by 50%: 1.5 M Beta vulgaris
NaCl concentration required to inhibit ATP-diphosphate exchange by 50%: 0.3 M Delonix regia
NaCl concentration required to inhibit ATP-diphosphate exchange by 50%: 0.27 M Parkinsonia aculeata
NaCl concentration required to inhibit ATP-diphosphate exchange by 50%: 1.43 M Phaseolus vulgaris
NH4Cl concentration required to inhibit ATP-diphosphate exchange by 50%: 1.0 M Beta vulgaris
NH4Cl concentration required to inhibit ATP-diphosphate exchange by 50%: 0.35 M Delonix regia
NH4Cl concentration required to inhibit ATP-diphosphate exchange by 50%: 0.38 M Parkinsonia aculeata
NH4Cl concentration required to inhibit ATP-diphosphate exchange by 50%: 1.1 M Phaseolus vulgaris
Pyrrole
-
 Delonix regia
Pyrrole
-
 Phaseolus vulgaris
Pyrrolidine
-
 Delonix regia
Pyrrolidine
-
 Phaseolus vulgaris
tetrahydrofuran
-
 Delonix regia
tetrahydrofuran
-
 Phaseolus vulgaris
Tetrahydrothiophen
-
 Delonix regia
Tetrahydrothiophen
-
 Phaseolus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.137
-
 L-Pro ATP-diphosphate exchange Phaseolus vulgaris
0.182
-
 L-Pro ATP-diphosphate exchange Delonix regia
0.28
-
 3,4-dehydro-DL-proline ATP-diphosphate exchange Phaseolus vulgaris
0.29
-
 L-Pro ATP-diphosphate exchange Ranunculus bulbosus
0.364
-
 3,4-dehydro-DL-proline ATP-diphosphate exchange Ranunculus bulbosus
0.43
-
 L-Pro ATP-diphosphate exchange Parkinsonia aculeata
0.45
-
 L-Pro ATP-diphosphate exchange Beta vulgaris
0.45
-
 L-Pro ATP-diphosphate exchange Convallaria majalis
0.5
-
 3,4-dehydro-DL-proline ATP-diphosphate exchange Beta vulgaris
0.625
-
 L-Pro ATP-diphosphate exchange Hemerocallis fulva
0.74
-
 3,4-dehydro-DL-proline ATP-diphosphate exchange Hemerocallis fulva
0.78
-
 3,4-dehydro-DL-proline ATP-diphosphate exchange Delonix regia
1.4
-
 3-thiaproline 3,4-dehydro-DL-proline, , ATP-diphosphate exchange Convallaria majalis
1.43
-
 L-azetidine-2-carboxylic acid ATP-diphosphate exchange Phaseolus vulgaris
2
-
 L-azetidine-2-carboxylic acid ATP-diphosphate exchange Ranunculus bulbosus
2 3 cis(exo)-3,4-Methano-L-proline ATP-diphosphate exchange Convallaria majalis
2.2
-
 3,4-dehydro-DL-proline ATP-diphosphate exchange Beta vulgaris
2.2
-
 3,4-dehydro-DL-proline ATP-diphosphate exchange, L-azetidine-2-carboxylic acid Parkinsonia aculeata
4.6
-
 cis(exo)-3,4-Methano-L-proline ATP-diphosphate exchange Delonix regia
5.3
-
 L-azetidine-2-carboxylic acid ATP-diphosphate exchange Hemerocallis fulva
7.1
-
 cis(exo)-3,4-Methano-L-proline ATP-diphosphate exchange Parkinsonia aculeata
20 50 L-thiazolidine-4-carboxylic acid ATP-diphosphate exchange Delonix regia
20
-
 thiazolidine-4-carboxylic acid ATP-diphosphate exchange Phaseolus vulgaris
45
-
 N-methylglycine ATP-diphosphate exchange Parkinsonia aculeata
67
-
 N-methylglycine ATP-diphosphate exchange Phaseolus vulgaris
68
-
 N-Methyl-L-alanine ATP-diphosphate exchange Phaseolus vulgaris
100
-
 N-methylglycine ATP-diphosphate exchange Hemerocallis fulva
140
-
 N-methylglycine ATP-diphosphate exchange Ranunculus bulbosus
300
-
 N-methylglycine approximate value, , ATP-diphosphate exchange Delonix regia

Organism

Organism UniProt Comment Textmining
Beta vulgaris
-
-
-
Convallaria majalis
-
-
-
Delonix regia
-
-
-
Hemerocallis fulva
-
-
-
Parkinsonia aculeata
-
-
-
Phaseolus vulgaris
-
-
-
Polygonatum multiflorum
-
-
-
Ranunculus bulbosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Phaseolus vulgaris
-
 Delonix regia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.101
-
-
 Delonix regia
1.655
-
-
 Delonix regia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3,4-dehydro-DL-proline + tRNAPro
-
 Phaseolus vulgaris ?
-
 ?
ATP + 3,4-dehydro-DL-proline + tRNAPro
-
 Delonix regia ?
-
 ?
ATP + 3,4-dehydro-DL-proline + tRNAPro
-
 Hemerocallis fulva ?
-
 ?
ATP + 3,4-dehydro-DL-proline + tRNAPro
-
 Ranunculus bulbosus ?
-
 ?
ATP + 3,4-dehydro-DL-proline + tRNAPro
-
 Beta vulgaris ?
-
 ?
ATP + 3,4-dehydro-DL-proline + tRNAPro
-
 Parkinsonia aculeata ?
-
 ?
ATP + 3-thiaproline + tRNAPro
-
 Convallaria majalis ?
-
 ?
ATP + cis(exo)-3,4-methano-L-proline + tRNAPro
-
 Delonix regia ?
-
 ?
ATP + cis(exo)-3,4-methano-L-proline + tRNAPro
-
 Parkinsonia aculeata ?
-
 ?
ATP + cis(exo)-3,4-methano-L-proline + tRNAPro
-
 Convallaria majalis ?
-
 ?
ATP + L-azetidine-2-carboxylic acid + tRNAPro
-
 Phaseolus vulgaris ?
-
 ?
ATP + L-azetidine-2-carboxylic acid + tRNAPro
-
 Hemerocallis fulva ?
-
 ?
ATP + L-azetidine-2-carboxylic acid + tRNAPro
-
 Ranunculus bulbosus ?
-
 ?
ATP + L-proline + tRNAPro
-
 Polygonatum multiflorum AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro N-methylglycine can replace proline in ATP-diphosphate exchange Phaseolus vulgaris AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro N-methylglycine can replace proline in ATP-diphosphate exchange Delonix regia AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro N-methylglycine can replace proline in ATP-diphosphate exchange Hemerocallis fulva AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro N-methylglycine can replace proline in ATP-diphosphate exchange Ranunculus bulbosus AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro N-methylglycine can replace proline in ATP-diphosphate exchange Beta vulgaris AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro N-methylglycine can replace proline in ATP-diphosphate exchange Parkinsonia aculeata AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro N-methylglycine can replace proline in ATP-diphosphate exchange Convallaria majalis AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro L-azetidine-2-carboxylic acid can replace proline in ATP-diphosphate exchange (not) Phaseolus vulgaris AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro L-azetidine-2-carboxylic acid can replace proline in ATP-diphosphate exchange (not) Hemerocallis fulva AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro L-azetidine-2-carboxylic acid can replace proline in ATP-diphosphate exchange (not) Ranunculus bulbosus AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro L-azetidine-2-carboxylic acid can replace proline in ATP-diphosphate exchange (not) Beta vulgaris AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro cis(exo)-3,4-methano-L-proline can replace proline on ATP-diphosphate exchange Delonix regia AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro cis(exo)-3,4-methano-L-proline can replace proline on ATP-diphosphate exchange Parkinsonia aculeata AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro cis(exo)-3,4-methano-L-proline can replace proline on ATP-diphosphate exchange Convallaria majalis AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange Phaseolus vulgaris AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange Delonix regia AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange Hemerocallis fulva AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange Ranunculus bulbosus AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange Beta vulgaris AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange Parkinsonia aculeata AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange Convallaria majalis AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro L-thiazolidine-4-carboxylic acid can replace proline in ATP-diphosphate exchange Phaseolus vulgaris AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro L-thiazolidine-4-carboxylic acid can replace proline in ATP-diphosphate exchange Delonix regia AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro L-thiazolidine-4-carboxylic acid can replace proline in ATP-diphosphate exchange Ranunculus bulbosus AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro L-thiazolidine-4-carboxylic acid can replace proline in ATP-diphosphate exchange Beta vulgaris AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro L-thiazolidine-4-carboxylic acid can replace proline in ATP-diphosphate exchange Parkinsonia aculeata AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-thiazolidine-4-carboxylic acid + tRNAPro
-
 Delonix regia ?
-
 ?
ATP + N-methyl-L-alanine + tRNAPro
-
 Phaseolus vulgaris ?
-
 ?
ATP + N-methylglycine + tRNAPro
-
 Phaseolus vulgaris ?
-
 ?
ATP + N-methylglycine + tRNAPro
-
 Delonix regia ?
-
 ?
ATP + N-methylglycine + tRNAPro
-
 Hemerocallis fulva ?
-
 ?
ATP + N-methylglycine + tRNAPro
-
 Ranunculus bulbosus ?
-
 ?
ATP + N-methylglycine + tRNAPro
-
 Parkinsonia aculeata ?
-
 ?
ATP + thiazolidine-4-carboxylic acid + tRNAPro
-
 Phaseolus vulgaris ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 50% loss of ATP-diphosphate exchange activity after 5 min, without stabilizing reagent Delonix regia
25
-
 50% loss of ATP-diphosphate exchange activity after 5 min, without stabilizing reagent Parkinsonia aculeata
51
-
 50% loss of ATP-diphosphate exchange activity after 5 min, without stabilizing reagent Ranunculus bulbosus
55
-
 50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.002 mM ATP Parkinsonia aculeata
56
-
 50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.002 mM ATP Delonix regia
58
-
 50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.02 mM Pro Parkinsonia aculeata
59
-
 50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.02 mM Pro Delonix regia
65
-
 50% loss of ATP-diphosphate exchange activity after 5 min, without stabilizing reagent Phaseolus vulgaris
67
-
 50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.002 mM ATP + 0.02 mM Pro Delonix regia
67
-
 50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.002 mM ATP + 0.02 mM Pro Parkinsonia aculeata
73
-
 50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.002 mM ATP Phaseolus vulgaris
78
-
 50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.002 mM ATP + 0.02 mM Pro Phaseolus vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 7.8 ATP-diphosphate exchange Phaseolus vulgaris
7.5 7.8 ATP-diphosphate exchange Ranunculus bulbosus
7.6 7.9 ATP-diphosphate exchange Hemerocallis fulva
7.9 8.3 ATP-diphosphate exchange Beta vulgaris
8.4 8.7 ATP-diphosphate exchange Convallaria majalis
8.4 8.8 ATP-diphosphate exchange Polygonatum multiflorum
8.8 9.2 ATP-diphosphate exchange Parkinsonia aculeata
9 9.4 ATP-diphosphate exchange Delonix regia