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Literature summary for 6.1.1.14 extracted from
- One of two genes encoding glycyl-tRNA synthetase in Saccharomyces cerevisiae provides mitochondrial and cytoplasmic functions (2000), J. Biol. Chem., 275, 27681-27688.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| 2 genes: grs1 encodes mitochondrial and cytoplasmic isozyme, grs2 encodes a mitochondrial dipensable isozyme, expression in Escherichia coli as HIs-tagged proteins, both cytoplasmic and mitochondrial phenotypes of a knockout allele of grs1 are complemented by the expression of the single gene of Schizosaccharomyces pombe, expression of gene grs2 in a grs1-deficient yeast mutant cannot complement the lethal phenotype | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + glycine + tRNAGly | Saccharomyces cerevisiae | - |
AMP + diphosphate + glycyl-tRNAGly | - |
? |  |
| additional information | Saccharomyces cerevisiae | phylogenetic analysis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
2 genes grs1 and grs2 encoding GlyRS1, a mitochondrial and cytoplasmic isozyme, and GlyRS2, a dispensable mitochondrial isozyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged isozymes GlyRS1 and 2 from Escherichia coli | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + glycine + tRNAGly | - |
Saccharomyces cerevisiae | AMP + diphosphate + glycyl-tRNAGly | - |
? | |
| additional information | phylogenetic analysis | Saccharomyces cerevisiae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Glycine--tRNA ligase | - |
Saccharomyces cerevisiae |
| Glycyl translase | - |
Saccharomyces cerevisiae |
| Glycyl-transfer ribonucleate synthetase | - |
Saccharomyces cerevisiae |
| Glycyl-transfer ribonucleic acid synthetase | - |
Saccharomyces cerevisiae |
| Glycyl-transfer RNA synthetase | - |
Saccharomyces cerevisiae |
| Glycyl-tRNA synthetase | - |
Saccharomyces cerevisiae |
| GlyRS | - |
Saccharomyces cerevisiae |
| Synthetase, glycyl-transfer ribonucleate | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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