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Literature summary for 6.1.1.14 extracted from

  • Kawakami, M; Nishio, K.
    Subunit structure and tRNA-binding properties of Bombyx mori glycyl-tRNA synthetase (1985), J. Biochem., 98, 177-186.
    View publication on PubMed
Search for more literature for 6.1.1.14

General Stability

General Stability Organism
DTT or 25% glycerol stabilizes Bombyx mori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 tRNAGly tRNA2Gly, , aminoacylation Bombyx mori
0.0026
-
 tRNA1Gly aminoacylation Bombyx mori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 2 * 90000, SDS-PAGE Bombyx mori
230000
-
 gel filtration Bombyx mori
270000
-
 polyacrylamide 4/30% gradient PAGE Bombyx mori

Organism

Organism UniProt Comment Textmining
Bombyx mori
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bombyx mori

Source Tissue

Source Tissue Comment Organism Textmining
silk gland posterior Bombyx mori
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.65
-
-
 Bombyx mori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glycine + tRNAGly
-
 Bombyx mori AMP + diphosphate + glycyl-tRNAGly
-
 ?
additional information enzyme has two active sites for aminoacylation. tRNA2Gly binds with the enzyme in a molar ratio of 1:1. tRNA2Gly forms a 2:1 complex with the enzyme and is aminoacylated 2fold faster Bombyx mori ?
-
 ?

Subunits

Subunits Comment Organism
? x * 87000-90000, SDS-PAGE, gel filtration in 6 M guanidine-HCl Bombyx mori
dimer 2 * 90000, SDS-PAGE Bombyx mori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.1
-
 tRNA2Gly aminoacylation Bombyx mori
15.9
-
 tRNA1Gly aminoacylation Bombyx mori