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Literature summary for 6.1.1.12 extracted from

  • Songsiriritthigul, C.; Suebka, S.; Chen, C.J.; Fuengfuloy, P.; Chuawong, P.
    Crystal structure of the N-terminal anticodon-binding domain of the nondiscriminating aspartyl-tRNA synthetase from Helicobacter pylori (2017), Acta Crystallogr. Sect. F, 73, 62-69 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Helicobacter pylori

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 7% (w/v) PEG 4000, 0.1 M ammonium sulfate, 0.1 M sodium citrate pH 5.6 Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate + tRNAAsp Helicobacter pylori
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
ATP + L-aspartate + tRNAAsp Helicobacter pylori ATCC 700392
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?

Organism

Organism UniProt Comment Textmining
Helicobacter pylori P56459
-
-
Helicobacter pylori ATCC 700392 P56459
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, and Sephacryl S200 gel filtration Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + tRNAAsp
-
 Helicobacter pylori AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
ATP + L-aspartate + tRNAAsp
-
 Helicobacter pylori ATCC 700392 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?

Synonyms

Synonyms Comment Organism
Aspartyl-tRNA synthetase
-
 Helicobacter pylori
AspRS
-
 Helicobacter pylori

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Helicobacter pylori