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Literature summary for 6.1.1.12 extracted from

  • Neuenfeldt, A.; Lorber, B.; Ennifar, E.; Gaudry, A.; Sauter, C.; Sissler, M.; Florentz, C.
    Thermodynamic properties distinguish human mitochondrial aspartyl-tRNA synthetase from bacterial homolog with same 3D architecture (2013), Nucleic Acids Res., 41, 2698-2708.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Rosetta 2 cells Homo sapiens
expressed in Escherichia coli JM83 cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion method, using 10% (m/v) PEG-3350 and 0.5M of ammonium sulfate in 25 mM Bis-Tris pH 5.5 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
tRNAAsp recombinant enzyme, at pH 7.5 and 25°C Escherichia coli
0.0011
-
tRNAAsp native enzyme, at pH 7.5 and 25°C Homo sapiens
0.0012
-
tRNAAsp recombinant enzyme, at pH 7.5 and 25°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate + tRNAAsp Escherichia coli
-
AMP + diphosphate + L-aspartyl-tRNAAsp
-
?
ATP + L-aspartate + tRNAAsp Homo sapiens
-
AMP + diphosphate + L-aspartyl-tRNAAsp
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens Q6PI48
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli
Ni-NTA column chromatography and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + tRNAAsp
-
Escherichia coli AMP + diphosphate + L-aspartyl-tRNAAsp
-
?
ATP + L-aspartate + tRNAAsp
-
Homo sapiens AMP + diphosphate + L-aspartyl-tRNAAsp
-
?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Escherichia coli
homodimer x-ray crystallography Homo sapiens

Synonyms

Synonyms Comment Organism
Aspartyl-tRNA synthetase
-
Escherichia coli
Aspartyl-tRNA synthetase
-
Homo sapiens
DRS
-
Escherichia coli
Drs2
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.004
-
tRNAAsp native enzyme, at pH 7.5 and 25°C Homo sapiens
0.0103
-
tRNAAsp recombinant enzyme, at pH 7.5 and 25°C Homo sapiens
0.23
-
tRNAAsp recombinant enzyme, at pH 7.5 and 25°C Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli
ATP
-
Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.7
-
tRNAAsp native enzyme, at pH 7.5 and 25°C Homo sapiens
8.3
-
tRNAAsp recombinant enzyme, at pH 7.5 and 25°C Homo sapiens
293
-
tRNAAsp recombinant enzyme, at pH 7.5 and 25°C Escherichia coli