Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Rosetta 2 cells | Homo sapiens |
expressed in Escherichia coli JM83 cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
vapor diffusion method, using 10% (m/v) PEG-3350 and 0.5M of ammonium sulfate in 25 mM Bis-Tris pH 5.5 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
tRNAAsp | recombinant enzyme, at pH 7.5 and 25°C | Escherichia coli | |
0.0011 | - |
tRNAAsp | native enzyme, at pH 7.5 and 25°C | Homo sapiens | |
0.0012 | - |
tRNAAsp | recombinant enzyme, at pH 7.5 and 25°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | Escherichia coli | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | Homo sapiens | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | Q6PI48 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Ni-NTA column chromatography and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | - |
Escherichia coli | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | - |
Homo sapiens | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Escherichia coli |
homodimer | x-ray crystallography | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Aspartyl-tRNA synthetase | - |
Escherichia coli |
Aspartyl-tRNA synthetase | - |
Homo sapiens |
DRS | - |
Escherichia coli |
Drs2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
tRNAAsp | native enzyme, at pH 7.5 and 25°C | Homo sapiens | |
0.0103 | - |
tRNAAsp | recombinant enzyme, at pH 7.5 and 25°C | Homo sapiens | |
0.23 | - |
tRNAAsp | recombinant enzyme, at pH 7.5 and 25°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli | |
ATP | - |
Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | - |
tRNAAsp | native enzyme, at pH 7.5 and 25°C | Homo sapiens | |
8.3 | - |
tRNAAsp | recombinant enzyme, at pH 7.5 and 25°C | Homo sapiens | |
293 | - |
tRNAAsp | recombinant enzyme, at pH 7.5 and 25°C | Escherichia coli |