Cloned (Comment) | Organism |
---|---|
expression of His6-tagged or GST-tagged enzyme in Escherichia coli strain BL21(DE3), and expression of biotin- or His6-tagged DRS-SUMO and DRS-ubiquitin fusion proteins in Escherichia coli strain BL21(DE3), since the free DRS is expressed as an inactive and insoluble protein in Escherichia coli, SUMO is a small ubiquitin-like modifier molecule | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | Homo sapiens | - |
AMP + diphosphate + aspartyl-tRNAAsp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged DRS-SUMO and DRS-ubiquitin fusion proteins by nickel affinity chromatography from Escherichia coli strain BL21(DE3), recombinant biotin-tagged DRS-ubiquitin fusion protein by avidin affinity chromatography from Escherichia coli strain Bl21(DE3) | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.7 | - |
purified recombinant His6-tagged DRS enzyme | Homo sapiens |
10.9 | - |
purified recombinant His6-tagged DRS-ubiquitin fusion enzyme | Homo sapiens |
11.5 | - |
purified recombinant biotin-tagged DRS-ubiquitin fusion enzyme | Homo sapiens |
13.5 | - |
purified recombinant His6-tagged DRS-small ubiquitin-like modifier fusion enzyme | Homo sapiens |
17 | - |
purified recombinant GST-tagged DRS enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | - |
Homo sapiens | AMP + diphosphate + aspartyl-tRNAAsp | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Aspartyl-tRNA synthetase | - |
Homo sapiens |
DRS | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |