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Literature summary for 6.1.1.12 extracted from

  • Eriani, G.; Gangloff, J.
    Yeast aspartyl-tRNA synthetase residues interacting with tRNA(Asp) identity bases connectively contribute to tRNA(Asp) binding in the ground and transition-state complex and discriminate against non-cognate tRNAs (1999), J. Mol. Biol., 291, 761-773.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of wild-type and mutants Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D210A central core binding mutant, increased acylation activity, increased dissociation constant Saccharomyces cerevisiae
E188A anticodon loop binding mutant, slightly reduced acylation activity, increased dissociation constant Saccharomyces cerevisiae
E188A/S239A anticodon-G73 binding mutant, reduced acylation activity, highly increased dissociation constant Saccharomyces cerevisiae
E188A/T331A anticodon-G73 binding mutant, highly reduced acylation activity, highly increased dissociation constant Saccharomyces cerevisiae
E202A central core binding mutant, slightly increased acylation activity, increased dissociation constant Saccharomyces cerevisiae
E327A acceptor arm binding mutant, reduced acylation activity Saccharomyces cerevisiae
F127A anticodon loop binding mutant, increased acylation activity, increased dissociation constant Saccharomyces cerevisiae
F127A/D210A anticodon binding mutant, reduced acylation activity, highly increased dissociation constant Saccharomyces cerevisiae
F127A/E188A anticodon binding mutant, reduced acylation activity, highly increased dissociation constant Saccharomyces cerevisiae
F127A/S239A anticodon-G73 binding mutant, reduced acylation activity, highly increased dissociation constant Saccharomyces cerevisiae
F127A/T331A anticodon-G73 binding mutant, highly reduced acylation activity, highly increased dissociation constant Saccharomyces cerevisiae
F304A terminal A binding mutant, reduced acylation activity Saccharomyces cerevisiae
H334A acceptor arm binding mutant, highly reduced acylation activity, increased dissociation constant Saccharomyces cerevisiae
K142A anticodon loop binding mutant, increased acylation activity, increased dissociation constant Saccharomyces cerevisiae
K142A/E188A anticodon binding mutant, reduced acylation activity, highly increased dissociation constant Saccharomyces cerevisiae
K155A central core binding mutant, increased acylation activity, increased dissociation constant Saccharomyces cerevisiae
K180A anticodon loop binding mutant, slightly increased acylation activity, increased dissociation constant Saccharomyces cerevisiae
K293A acceptor arm binding mutant, highly increased acylation activity, increased dissociation constant Saccharomyces cerevisiae
K428A acceptor arm binding mutant, increased acylation activity, increased dissociation constant Saccharomyces cerevisiae
K553A acceptor arm binding mutant, acylation activity similar to wild-type, increased dissociation constant Saccharomyces cerevisiae
additional information cooperativity analysis of the G73 and anticodon binding interactions, overview, effect of mutations on cell growth Saccharomyces cerevisiae
N117A anticodon loop binding mutant, increased acylation activity, increased dissociation constant Saccharomyces cerevisiae
N227A central core binding mutant, slightly increased acylation activity, increased dissociation constant Saccharomyces cerevisiae
N328A acceptor arm binding mutant, reduced acylation activity, increased dissociation constant Saccharomyces cerevisiae
N328A/S329A/T331A G73 binding mutant, highly reduced acylation activity, increased dissociation constant Saccharomyces cerevisiae
Q121A anticodon loop binding mutant, increased acylation activity, increased dissociation constant Saccharomyces cerevisiae
Q138A anticodon loop binding mutant, slightly increased acylation activity, increased dissociation constant Saccharomyces cerevisiae
Q138A/E188A anticodon binding mutant, reduced acylation activity, highly increased dissociation constant Saccharomyces cerevisiae
Q138A/R119A anticodon binding mutant, reduced acylation activity, highly increased dissociation constant Saccharomyces cerevisiae
Q300A terminal A binding mutant, reduced acylation activity Saccharomyces cerevisiae
R119A anticodon loop binding mutant, increased acylation activity, increased dissociation constant Saccharomyces cerevisiae
R119A/E188A anticodon binding mutant, highly reduced acylation activity, highly increased dissociation constant Saccharomyces cerevisiae
S181A anticodon loop binding mutant, increased acylation activity, increased dissociation constant Saccharomyces cerevisiae
S280A terminal A binding mutant, reduced acylation activity Saccharomyces cerevisiae
S301A terminal A binding mutant, reduced acylation activity, slightly increased dissociation constant Saccharomyces cerevisiae
S329A acceptor arm binding mutant, increased acylation activity, increased dissociation constant Saccharomyces cerevisiae
S423A acceptor arm binding mutant, reduced acylation activity Saccharomyces cerevisiae
T124A anticodon loop binding mutant, slightly reduced acylation activity, increased dissociation constant Saccharomyces cerevisiae
T230A central core binding mutant, increased acylation activity, increased dissociation constant Saccharomyces cerevisiae
T331A acceptor arm binding mutant, reduced acylation activity, increased dissociation constant Saccharomyces cerevisiae
T424A acceptor arm binding mutant, acylation activity similar to the wild-type, increased dissociation constant Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate + tRNAAsp Saccharomyces cerevisiae
-
AMP + diphosphate + L-aspartyl-tRNAAsp
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
recombinant purified enzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutants Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
ATP + L-aspartate + tRNAAsp = AMP + diphosphate + L-aspartyl-tRNAAsp mechanism Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + tRNAAsp
-
Saccharomyces cerevisiae AMP + diphosphate + L-aspartyl-tRNAAsp
-
?

Synonyms

Synonyms Comment Organism
Aspartic acid translase
-
Saccharomyces cerevisiae
Aspartyl ribonucleate synthetase
-
Saccharomyces cerevisiae
aspartyl ribonuleic synthetase
-
Saccharomyces cerevisiae
Aspartyl-transfer ribonucleic acid synthetase
-
Saccharomyces cerevisiae
Aspartyl-transfer RNA synthetase
-
Saccharomyces cerevisiae
Aspartyl-tRNA synthetase
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.3
-
tRNAAsp wild-type enzyme, acylation activity, pH 7.5, 37°C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Saccharomyces cerevisiae