Cloned (Comment) | Organism |
---|---|
aspS1 gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strains DH5alpha and BL21 | Thermus thermophilus |
aspS2 gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strains DH5alpha and BL21 | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00003 | - |
tRNAAsp | pH 7.2, 70°C | Thermus thermophilus | |
0.000063 | - |
tRNAAsn | pH 7.2, 70°C | Thermus thermophilus | |
0.000073 | - |
tRNAAsp | pH 7.2, 70°C | Thermus thermophilus | |
0.0034 | - |
tRNAAsn | pH 7.2, 70°C | Thermus thermophilus | |
0.005 | - |
L-aspartic acid | pH 7.2, 70°C | Thermus thermophilus | |
0.03 | - |
aspartic acid | pH 7.2, 70°C | Thermus thermophilus | |
0.033 | - |
ATP | pH 7.2, 70°C | Thermus thermophilus | |
0.28 | - |
ATP | pH 7.2, 70°C | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48329 | - |
x * 48329, amino acid sequence determination | Thermus thermophilus |
66029 | - |
x * 66029, amino acid sequence determination | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | Thermus thermophilus | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | P36419 | Asp-tRNA synthetase AspRS1 | - |
Thermus thermophilus | Q5SIC2 | Asp-tRNA synthetase AspRS2 | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | P36419 | Asp-tRNA synthetase AspRS1 | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q5SIC2 | Asp-tRNA synthetase AspRS2 | - |
Purification (Comment) | Organism |
---|---|
recombinant from overexpressing Escherichia coli, 34.3fold | Thermus thermophilus |
recombinant from overexpressing Escherichia coli, 7.4fold | Thermus thermophilus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-aspartate + tRNAAsp = AMP + diphosphate + L-aspartyl-tRNAAsp | conserved amino acid residues of functional importance in consensus motifs: Pro227 in motif 1, Arg332 and Glu334 in motif 2, Gly675, Gly679, Asp681, Arg682, and Pro700 in motif 3 | Thermus thermophilus | |
ATP + L-aspartate + tRNAAsp = AMP + diphosphate + L-aspartyl-tRNAAsp | conserved amino acid residues of functional importance in consensus motifs: Pro227 in motif 1, Arg332 and Glu334 in motif 2, Gly675, Gly679, Glu681, Arg682, and Pro700 in motif 3 | Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00002 | - |
native enzyme, cell extract, 37°C | Thermus thermophilus |
0.000083 | - |
native enzyme, cell extract, 37°C | Thermus thermophilus |
0.0336 | - |
purified recombinant enzyme, 37°C | Thermus thermophilus |
0.043 | - |
recombinant enzyme, Escherichia coli DH5alpha cell extract, 37°C | Thermus thermophilus |
0.055 | - |
recombinant enzyme, Escherichia coli BL21 cell extract, 37°C | Thermus thermophilus |
0.124 | - |
recombinant enzyme, Escherichia coli BL21 cell extract, 37°C | Thermus thermophilus |
0.155 | - |
recombinant enzyme, Escherichia coli DH5alpha cell extract, 37°C | Thermus thermophilus |
0.321 | - |
purified recombinant enzyme, 37°C | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsn | about half as effective as tRNAAsp | Thermus thermophilus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsn | about have as effective as tRNAAsp | Thermus thermophilus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsn | about half as effective as tRNAAsp | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsn | about have as effective as tRNAAsp | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | - |
Thermus thermophilus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | Thermus thermophilus or Escherichia coli tRNA | Thermus thermophilus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | Thermus thermophilus or Eschrichia coli tRNA | Thermus thermophilus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | Thermus thermophilus or Escherichia coli tRNA | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | Thermus thermophilus or Eschrichia coli tRNA | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48329, amino acid sequence determination | Thermus thermophilus |
? | x * 66029, amino acid sequence determination | Thermus thermophilus |
More | modular organization | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
Aspartic acid translase | - |
Thermus thermophilus |
Aspartyl ribonucleate synthetase | - |
Thermus thermophilus |
aspartyl ribonuleic synthetase | - |
Thermus thermophilus |
Aspartyl-transfer ribonucleic acid synthetase | - |
Thermus thermophilus |
Aspartyl-transfer RNA synthetase | - |
Thermus thermophilus |
Aspartyl-tRNA synthetase | - |
Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.092 | - |
tRNAAsn | pH 7.2, 70°C | Thermus thermophilus | |
0.12 | - |
tRNAAsn | pH 7.2, 70°C | Thermus thermophilus | |
0.24 | - |
tRNAAsp | pH 7.2, 70°C | Thermus thermophilus | |
2.7 | - |
tRNAAsp | pH 7.2, 70°C | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Thermus thermophilus |