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Literature summary for 6.1.1.12 extracted from

  • Vellekamp, G.J.; Coyle, C.L.; Kull, F.J.
    Low molecular weight aspartyl-tRNA synthetase from porcine thyroid. Purification, characterization, and heterogeneity (1983), J. Biol. Chem., 258, 8195-8200.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Br-
-
Sus scrofa
CaCl2 1 mM, more than 50% inhibition Sus scrofa
Cl-
-
Sus scrofa
F-
-
Sus scrofa
I-
-
Sus scrofa
NiCl2 0.1 mM more than 50% inhibition Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000061
-
tRNAAsp
-
Sus scrofa
0.067
-
Asp
-
Sus scrofa
0.26
-
ATP
-
Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Sus scrofa
Mg2+ optimal Mg2+/ATP ratio is 2 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
2 * 53000, gel electrophoresis under denaturing conditions Sus scrofa
124000
-
gel filtration Sus scrofa
156000
-
nondenaturing PAGE Sus scrofa

Organic Solvent Stability

Organic Solvent Comment Organism
Glycerol 25% v/v, required throughout purification procedure to maintain stability Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme form PC-1 (in addition 3 low MW enzyme forms exist: PC-2a, PC-2b and PC-2c) Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
thyroid gland
-
Sus scrofa
-

Storage Stability

Storage Stability Organism
-20°C, 10 mM potassium phosphate, pH 7.6, 10 mM 2-mercaptoethanol, 50% glycerol, stable Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + tRNAAsp
-
Sus scrofa AMP + diphosphate + L-aspartyl-tRNAAsp
-
?

Subunits

Subunits Comment Organism
dimer 2 * 53000, gel electrophoresis under denaturing conditions Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8 potassium Hepes buffer Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.1
-
NiCl2
-
Sus scrofa
1
-
CaCl2
-
Sus scrofa