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Literature summary for 6.1.1.12 extracted from

  • Agou, F.; Waller, J.P.; Mirande, M.
    Expression of rat aspartyl-tRNA synthetase in Saccharomyces cerevisiae. Role of the NH2-terminal polypeptide extension on enzyme activity and stability (1996), J. Biol. Chem., 271, 29295-29303.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae, wild-type enzyme and two truncated forms lacking 20 or 36 amino acid residues from their amino-terminal polypeptide extension Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information two truncated forms lacking 20 or 36 amino acid residues from their amino-terminal polypeptide extension, expressed in Saccharomyces cerevisiae, are less stable than wild-type enzyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
tRNAAsp aspartyl-tRNA synthetase as a component of the multisynthetase complex or recombinant native enzyme Rattus norvegicus
0.0006
-
tRNAAsp tRNAAsp, , truncated enzymes AspRS-DELTAN36 and AspRS-DELTAN20 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Mammalia
-
-
-
Rattus norvegicus
-
wild-type and two truncated forms lacking 20 or 36 amino acid residues from their amino-terminal polypeptide extension, overexpressed in Saccharomyces cerevisiae
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and two truncated forms Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.395
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + tRNAAsp
-
Mammalia AMP + diphosphate + L-aspartyl-tRNAAsp
-
?
ATP + L-aspartate + tRNAAsp
-
Rattus norvegicus AMP + diphosphate + L-aspartyl-tRNAAsp
-
?

Subunits

Subunits Comment Organism
More enzyme occurs in the multienzyme complex of aminoacyl-tRNA synthetases Mammalia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Rattus norvegicus