General Stability | Organism |
---|---|
glycerol stabilizes at low pH | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
L-Ser | pH 8.8 | Escherichia coli | |
0.07 | - |
L-Ser | acylation | Escherichia coli | |
0.2 | - |
D-Ser | serine-dependent ATP-diphosphate exchange | Escherichia coli | |
0.3 | - |
L-Ser | serine-dependent ATP-diphosphate exchange | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activity remains relatively unchanged between 5.2 and 24 mM | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
2 * 47000, sedimentation studies after dissociation in 6 M guanidine hydrochloride | Escherichia coli |
95000 | - |
sedimentation studies | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K12 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 10 mM potassium phosphate buffer, pH 6.8, containing 20 mM 2-mercaptoethanol, 0.5 mM EDTA and 10% glycerol | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-Ser + tRNASer | - |
Escherichia coli | ? | - |
? | |
ATP + L-serine + tRNASer | D-Ser can replace L-Ser in ATP-diphosphate exchange | Escherichia coli | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
additional information | serine-dependent ATP-diphosphate exchange | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 47000, sedimentation studies after dissociation in 6 M guanidine hydrochloride | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 8.8 | aminoacylation | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
glycerol stabilizes at low pH | Escherichia coli |
4 | 6 | 37°C, stability gradually decreases when pH is lowered from 6 to 4 | Escherichia coli |