Crystallization (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
EDTA, 10-50 mM, stabilizes purified enzyme at 2-4°C and at 37°C, no protective effect at 45°C | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00024 | - |
tRNASer | substrate from yeast | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimal Mg/ATP ratio is 1.5-2.5 | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
89000 | - |
equilibrium ultracentrifugation method | Saccharomyces cerevisiae |
114000 | - |
sucrose density gradient centrifugation | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.53 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + diphosphate + L-seryl-tRNASer | - |
Saccharomyces cerevisiae | ATP + L-serine + tRNASer | - |
r | |
ATP + L-serine + tRNASer | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-seryl-tRNASer | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
EDTA, 10-50 mM, stabilizes purified enzyme at 2-4°C and at 37°C, no protective effect at 45°C | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.833 | - |
L-seryl-tRNASer | - |
Saccharomyces cerevisiae |