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Literature summary for 6.1.1.11 extracted from
- Purification and properties of serine-activating enzyme from beef pancreas (1961), J. Biol. Chem., 236, 479-484.
General Stability
| General Stability | Organism |
|---|---|
| unstable to dialysis against 0.15 M KCl for 3 h at 4°C | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-hydroxymercuribenzoate | - |
Bos taurus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | metal ion required, optimal activity with 20 mM MnCl2 or MgCl2 | Bos taurus | |
| Mn2+ | metal ion required, optimal activity with 20 mM MnCl2 or MgCl2 | Bos taurus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.093 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 6 months, 50-75% of the original activity is retained | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-serine + tRNASer | - |
Bos taurus | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
hydroxamate assay | Bos taurus |
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Release 2023.1 (January 2023)
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