Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.11 extracted from

  • Xu, X.; Shi, Y.; Yang, X.L.
    Crystal structure of human seryl-tRNA synthetase and Ser-SA complex reveals a molecular lever specific to higher eukaryotes (2013), Structure, 21, 2078-2086.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with 5'-O-(N-(L-seryl)-sulfamoyl)adenosine, sitting drop vapor diffusion method, using 20% (w/v) PEG 3350, 0.2 M ammonium fluoride or ammonium formate, and 0.1 M HEPES pH 7.0 Homo sapiens

Protein Variants

Protein Variants Comment Organism
DELTAG254-N261 the mutant shows 72% activity compared to the wild type enzyme Homo sapiens
DELTAG75-N97 the mutant shows 13% activity compared to the wild type enzyme Homo sapiens
DELTAG75-N97/DELTAG254-N261 the mutant shows 7% activity compared to the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00013
-
 tRNASer mutant enzyme DELTAG75-N97/DELTAG254-N261, at pH 7.5 and 37°C Homo sapiens
0.00033
-
 tRNASer mutant enzyme DELTAG75-N97, at pH 7.5 and 37°C Homo sapiens
0.00051
-
 tRNASer mutant enzyme DELTAG254-N261, at pH 7.5 and 37°C Homo sapiens
0.00077
-
 tRNASer wild type enzyme, at pH 7.5 and 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-serine + tRNASer Homo sapiens
-
 AMP + diphosphate + L-seryl-tRNASer
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P49591
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-serine + tRNASer
-
 Homo sapiens AMP + diphosphate + L-seryl-tRNASer
-
 ?

Synonyms

Synonyms Comment Organism
SerRS
-
 Homo sapiens
Seryl-tRNA synthetase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 tRNASer mutant enzyme DELTAG75-N97/DELTAG254-N261, at pH 7.5 and 37°C Homo sapiens
0.025
-
 tRNASer mutant enzyme DELTAG75-N97, at pH 7.5 and 37°C Homo sapiens
0.2
-
 tRNASer mutant enzyme DELTAG254-N261, at pH 7.5 and 37°C Homo sapiens
0.43
-
 tRNASer wild type enzyme, at pH 7.5 and 37°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function seryl-tRNA synthetase is an essential enzyme for translation, also regulating vascular development Homo sapiens