Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 (DE3) cells | Methanopyrus kandleri |
Crystallization (Comment) | Organism |
---|---|
in complex with tRNASec from Aquifex aeolicus, sitting drop vapor diffusion method, using 100 mM trisodium citrate-HCl buffer pH 5.6 containing 2.0 M ammonium sulfate and 200 mM potassium sodium tartrate | Methanopyrus kandleri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | Methanopyrus kandleri | - |
AMP + diphosphate + L-seryl-tRNASer | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanopyrus kandleri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Toyopearl phenyl 650 column chromatography | Methanopyrus kandleri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | - |
Methanopyrus kandleri | AMP + diphosphate + L-seryl-tRNASer | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Methanopyrus kandleri |
Synonyms | Comment | Organism |
---|---|---|
SerRS | - |
Methanopyrus kandleri |
Seryl-tRNA synthetase | - |
Methanopyrus kandleri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Methanopyrus kandleri |