Literature summary for 6.1.1.11 extracted from

Artero, J.B.; Teixeira, S.C.; Mitchell, E.P.; Kron, M.A.; Forsyth, V.T.; Haertlein, M.
Crystallization and preliminary X-ray diffraction analysis of human cytosolic seryl-tRNA synthetase (2010), Acta Crystallogr. Sect. F, 66, 1521-1524.

Cloned(Commentary)

Cloned (Comment)	Organism
hsSerRS DNA and amino acid sequence determination, analysis, and comparisons, expression as His-tagged enzyme in Escherichia coli coli BL21 (DE3)	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified hsSerRS, sitting-drop vapour diffusion method, 0.01875 ml of protein solution containing 10 mg/ml protein in HCl, pH 7.9, 100 mM NaCl, 10 mM MgCl2, 5% glycerol, 5 mM DTT, with 0.00625 ml of reservoir solution containing 100 mM ammonium sulfate, 22% w/v PEG 3350, 5% glycerol, 200 mM sodium formate pH 7.2, supplemented with 20% v/v glycerol, and equilibration against 0.5 ml of reservoir solution, 12°C, 2 weeks, X-ray diffraction structure determination and analysis at 3.1 A resolution	Homo sapiens

Crystallization (Comment)

Organism

purified hsSerRS, sitting-drop vapour diffusion method, 0.01875 ml of protein solution containing 10 mg/ml protein in HCl, pH 7.9, 100 mM NaCl, 10 mM MgCl2, 5% glycerol, 5 mM DTT, with 0.00625 ml of reservoir solution containing 100 mM ammonium sulfate, 22% w/v PEG 3350, 5% glycerol, 200 mM sodium formate pH 7.2, supplemented with 20% v/v glycerol, and equilibration against 0.5 ml of reservoir solution, 12°C, 2 weeks, X-ray diffraction structure determination and analysis at 3.1 A resolution

Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Homo sapiens	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-serine + tRNASer	Homo sapiens	-	AMP + diphosphate + L-seryl-tRNASer	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged hsSerRS from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-serine + tRNASer	-	Homo sapiens	AMP + diphosphate + L-seryl-tRNASer	-	?
additional information	structure-function analysis, overview	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
SerRS	-	Homo sapiens
Seryl-tRNA synthetase	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

General Information

General Information	Comment	Organism
evolution	as a consequence of an N-terminal insertion and a C-terminal extra-sequence, the binding mode of tRNASer to hsSerRS differs from that in prokaryotes	Homo sapiens