Cloned (Comment) | Organism |
---|---|
the vectors pET15b, pET20b, and pET28b are used for expression of the proteins in Escherichia coli BL21 cells, the vectors pACT2 and pAB151 are used in yeast two-hybrid analysis | Methanosarcina barkeri |
Protein Variants | Comment | Organism |
---|---|---|
E273A | E273A replacement in helix 9 located in the C-terminal domain | Methanosarcina barkeri |
E273A/D277A/K280A | triple replacement in helix 9 | Methanosarcina barkeri |
K164A | K164A replacement in the linker peptide | Methanosarcina barkeri |
mMbSerRS-CTD | START codon introduced at position 167, which generates the C-terminal domain | Methanosarcina barkeri |
mMbSerRS-CTDDELTAHTH | START codon introduced at position 167 of mMbSerRS-DELTAHTH, generating the C-terminal domain without the helix-turn-helix motif | Methanosarcina barkeri |
mMbSerRS-DELTAHTH | deleted helix-turn-helix motif | Methanosarcina barkeri |
mMbSerRS-NTD | STOP codon introduced at position 164, which generates the N-terminal domain | Methanosarcina barkeri |
R147A | R147A replacement in helix 4 located in the N-terminal domain | Methanosarcina barkeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
tRNASer | mutant K164A | Methanosarcina barkeri | |
0.0026 | - |
tRNASer | wild-type | Methanosarcina barkeri | |
0.0042 | - |
tRNASer | mutant E273A | Methanosarcina barkeri | |
0.0047 | - |
tRNASer | mutant E273A/D277A/K280A | Methanosarcina barkeri | |
0.0146 | - |
tRNASer | mutant R147A | Methanosarcina barkeri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Methanosarcina barkeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | Methanosarcina barkeri | - |
AMP + diphosphate + L-seryl-tRNASer | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | - |
Methanosarcina barkeri | AMP + diphosphate + L-seryl-tRNASer | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Methanosarcina barkeri |
Synonyms | Comment | Organism |
---|---|---|
methanogenic-type SerRS | - |
Methanosarcina barkeri |
mMbSerRS | - |
Methanosarcina barkeri |
SerRS | - |
Methanosarcina barkeri |
Seryl-tRNA synthetase | - |
Methanosarcina barkeri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0087 | - |
tRNASer | mutant K164A | Methanosarcina barkeri | |
0.15 | - |
tRNASer | mutant R147A | Methanosarcina barkeri | |
0.45 | - |
tRNASer | wild-type | Methanosarcina barkeri | |
0.55 | - |
tRNASer | mutant E273A | Methanosarcina barkeri | |
1.4 | - |
tRNASer | mutant E273A/D277A/K280A | Methanosarcina barkeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Methanosarcina barkeri |